1lti
From Proteopedia
(New page: 200px<br /><applet load="1lti" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lti, resolution 2.13Å" /> '''HEAT-LABILE ENTEROTO...) |
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| - | [[Image:1lti.jpg|left|200px]]<br /><applet load="1lti" size=" | + | [[Image:1lti.jpg|left|200px]]<br /><applet load="1lti" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lti, resolution 2.13Å" /> | caption="1lti, resolution 2.13Å" /> | ||
'''HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN'''<br /> | '''HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and | + | Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and consists of a catalytic A subunit and a B pentamer that serves to recognize the oligosaccharide part of the GM1 ganglioside receptor. We report here the crystal structure of heat-labile enterotoxin in complex with the disaccharide portion of the Thomsen-Friedenreich (T-antigen) tumor marker. The toxin:carbohydrate complex is determined to 2.13 A resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human carcinomas and monitoring its autoimmune response is used for the early detection of tumors. Insight into the molecular recognition of this tumor antigen by sugar binding proteins can benefit the development of a diagnostic tool for human carcinomas as well as a T-antigen directed anticancer drug delivery system. |
==About this Structure== | ==About this Structure== | ||
| - | 1LTI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LTI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Akker, F | + | [[Category: Akker, F Van Den.]] |
| - | [[Category: Hol, W | + | [[Category: Hol, W G.J.]] |
[[Category: GAL]] | [[Category: GAL]] | ||
[[Category: adp-ribosyl transferase]] | [[Category: adp-ribosyl transferase]] | ||
[[Category: enterotoxin]] | [[Category: enterotoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:19 2008'' |
Revision as of 11:48, 21 February 2008
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HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN
Overview
Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and consists of a catalytic A subunit and a B pentamer that serves to recognize the oligosaccharide part of the GM1 ganglioside receptor. We report here the crystal structure of heat-labile enterotoxin in complex with the disaccharide portion of the Thomsen-Friedenreich (T-antigen) tumor marker. The toxin:carbohydrate complex is determined to 2.13 A resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human carcinomas and monitoring its autoimmune response is used for the early detection of tumors. Insight into the molecular recognition of this tumor antigen by sugar binding proteins can benefit the development of a diagnostic tool for human carcinomas as well as a T-antigen directed anticancer drug delivery system.
About this Structure
1LTI is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Tumor marker disaccharide D-Gal-beta 1, 3-GalNAc complexed to heat-labile enterotoxin from Escherichia coli., van den Akker F, Steensma E, Hol WG, Protein Sci. 1996 Jun;5(6):1184-8. PMID:8762150
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