1lv7
From Proteopedia
(New page: 200px<br /><applet load="1lv7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lv7, resolution 1.50Å" /> '''Crystal Structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lv7.gif|left|200px]]<br /><applet load="1lv7" size=" | + | [[Image:1lv7.gif|left|200px]]<br /><applet load="1lv7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lv7, resolution 1.50Å" /> | caption="1lv7, resolution 1.50Å" /> | ||
'''Crystal Structure of the AAA domain of FtsH'''<br /> | '''Crystal Structure of the AAA domain of FtsH'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Eubacteria and eukaryotic cellular organelles have membrane-bound | + | Eubacteria and eukaryotic cellular organelles have membrane-bound ATP-dependent proteases, which degrade misassembled membrane protein complexes and play a vital role in membrane quality control. The bacterial protease FtsH also degrades an interesting subset of cytoplasmic regulatory proteins, including sigma(32), LpxC, and lambda CII. The crystal structure of the ATPase module of FtsH has been solved, revealing an alpha/beta nucleotide binding domain connected to a four-helix bundle, similar to the AAA modules of proteins involved in DNA replication and membrane fusion. A sulfate anion in the ATP binding pocket mimics the beta-phosphate group of an adenine nucleotide. A hexamer form of FtsH has been modeled, providing insights into possible modes of nucleotide binding and intersubunit catalysis. |
==About this Structure== | ==About this Structure== | ||
| - | 1LV7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LV7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV7 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brzozowski, A | + | [[Category: Brzozowski, A M.]] |
[[Category: Karata, K.]] | [[Category: Karata, K.]] | ||
[[Category: Krzywda, S.]] | [[Category: Krzywda, S.]] | ||
[[Category: Ogura, T.]] | [[Category: Ogura, T.]] | ||
[[Category: Verma, C.]] | [[Category: Verma, C.]] | ||
| - | [[Category: Wilkinson, A | + | [[Category: Wilkinson, A J.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: alpha/beta domain]] | [[Category: alpha/beta domain]] | ||
[[Category: four helix bundle]] | [[Category: four helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:48 2008'' |
Revision as of 11:48, 21 February 2008
|
Crystal Structure of the AAA domain of FtsH
Overview
Eubacteria and eukaryotic cellular organelles have membrane-bound ATP-dependent proteases, which degrade misassembled membrane protein complexes and play a vital role in membrane quality control. The bacterial protease FtsH also degrades an interesting subset of cytoplasmic regulatory proteins, including sigma(32), LpxC, and lambda CII. The crystal structure of the ATPase module of FtsH has been solved, revealing an alpha/beta nucleotide binding domain connected to a four-helix bundle, similar to the AAA modules of proteins involved in DNA replication and membrane fusion. A sulfate anion in the ATP binding pocket mimics the beta-phosphate group of an adenine nucleotide. A hexamer form of FtsH has been modeled, providing insights into possible modes of nucleotide binding and intersubunit catalysis.
About this Structure
1LV7 is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli at 1.5 A resolution., Krzywda S, Brzozowski AM, Verma C, Karata K, Ogura T, Wilkinson AJ, Structure. 2002 Aug;10(8):1073-83. PMID:12176385
Page seeded by OCA on Thu Feb 21 13:48:48 2008
