1lv0
From Proteopedia
(New page: 200px<br /><applet load="1lv0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lv0, resolution 2.00Å" /> '''Crystal structure of...) |
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| - | [[Image:1lv0.jpg|left|200px]]<br /><applet load="1lv0" size=" | + | [[Image:1lv0.jpg|left|200px]]<br /><applet load="1lv0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lv0, resolution 2.00Å" /> | caption="1lv0, resolution 2.00Å" /> | ||
'''Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide'''<br /> | '''Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rab GTPases, key regulators of membrane targeting and fusion, require the | + | Rab GTPases, key regulators of membrane targeting and fusion, require the covalent attachment of geranylgeranyl lipids to their C terminus for function. To elucidate the role of lipid in Rab recycling, we have determined the crystal structure of Rab guanine nucleotide dissociation inhibitor (alphaGDI) in complex with a geranylgeranyl (GG) ligand (H(2)N-Cys-(S-GG)-OMe). The lipid is bound beneath the Rab binding platform in a shallow hydrophobic groove. Mutation of the binding pocket in the brain-specific alphaGDI leads to mental retardation. Strikingly, lipid binding acts through a conserved allosteric switching mechanism to promote release of the GDI-Rab[GDP] complex from the membrane. |
==About this Structure== | ==About this Structure== | ||
| - | 1LV0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 and GER as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1LV0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GER:'>GER</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Alory, C.]] | [[Category: Alory, C.]] | ||
[[Category: An, Y.]] | [[Category: An, Y.]] | ||
| - | [[Category: Balch, W | + | [[Category: Balch, W E.]] |
[[Category: Chen, W.]] | [[Category: Chen, W.]] | ||
| - | [[Category: Gibbs, R | + | [[Category: Gibbs, R A.]] |
[[Category: Matteson, J.]] | [[Category: Matteson, J.]] | ||
[[Category: Sakisaka, T.]] | [[Category: Sakisaka, T.]] | ||
[[Category: Shao, Y.]] | [[Category: Shao, Y.]] | ||
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
[[Category: GER]] | [[Category: GER]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: protein-ligand complex]] | [[Category: protein-ligand complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:43 2008'' |
Revision as of 11:48, 21 February 2008
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Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide
Overview
Rab GTPases, key regulators of membrane targeting and fusion, require the covalent attachment of geranylgeranyl lipids to their C terminus for function. To elucidate the role of lipid in Rab recycling, we have determined the crystal structure of Rab guanine nucleotide dissociation inhibitor (alphaGDI) in complex with a geranylgeranyl (GG) ligand (H(2)N-Cys-(S-GG)-OMe). The lipid is bound beneath the Rab binding platform in a shallow hydrophobic groove. Mutation of the binding pocket in the brain-specific alphaGDI leads to mental retardation. Strikingly, lipid binding acts through a conserved allosteric switching mechanism to promote release of the GDI-Rab[GDP] complex from the membrane.
About this Structure
1LV0 is a Single protein structure of sequence from Bos taurus with and as ligands. Full crystallographic information is available from OCA.
Reference
Geranylgeranyl switching regulates GDI-Rab GTPase recycling., An Y, Shao Y, Alory C, Matteson J, Sakisaka T, Chen W, Gibbs RA, Wilson IA, Balch WE, Structure. 2003 Mar;11(3):347-57. PMID:12623022
Page seeded by OCA on Thu Feb 21 13:48:43 2008
Categories: Bos taurus | Single protein | Alory, C. | An, Y. | Balch, W E. | Chen, W. | Gibbs, R A. | Matteson, J. | Sakisaka, T. | Shao, Y. | Wilson, I A. | GER | SO4 | Protein-ligand complex
