1lvr
From Proteopedia
(New page: 200px<br /> <applet load="1lvr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lvr" /> '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i...) |
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| - | [[Image:1lvr.gif|left|200px]]<br /> | + | [[Image:1lvr.gif|left|200px]]<br /><applet load="1lvr" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lvr" size=" | + | |
caption="1lvr" /> | caption="1lvr" /> | ||
'''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i'''<br /> | '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the C-terminal region of the third cytoplasmic loop (IC3) | + | The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1LVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1LVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVR OCA]. |
==Reference== | ==Reference== | ||
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12237474 12237474] | Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12237474 12237474] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kendall, D | + | [[Category: Kendall, D A.]] |
| - | [[Category: McMurry, J | + | [[Category: McMurry, J L.]] |
| - | [[Category: Mierke, D | + | [[Category: Mierke, D F.]] |
[[Category: Miller, A.]] | [[Category: Miller, A.]] | ||
| - | [[Category: Ulfers, A | + | [[Category: Ulfers, A L.]] |
[[Category: Wang, L.]] | [[Category: Wang, L.]] | ||
[[Category: alpha domain of g protein i]] | [[Category: alpha domain of g protein i]] | ||
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[[Category: transferred noes]] | [[Category: transferred noes]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:57 2008'' |
Revision as of 11:48, 21 February 2008
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IC3 of CB1 (L431A,A432L) Bound to G(alpha)i
Overview
The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.
About this Structure
1LVR is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474
Page seeded by OCA on Thu Feb 21 13:48:57 2008
