This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lvr
From Proteopedia
(New page: 200px<br /> <applet load="1lvr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lvr" /> '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lvr.gif|left|200px]]<br /> | + | [[Image:1lvr.gif|left|200px]]<br /><applet load="1lvr" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lvr" size=" | + | |
caption="1lvr" /> | caption="1lvr" /> | ||
'''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i'''<br /> | '''IC3 of CB1 (L431A,A432L) Bound to G(alpha)i'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the C-terminal region of the third cytoplasmic loop (IC3) | + | The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1LVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1LVR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVR OCA]. |
==Reference== | ==Reference== | ||
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12237474 12237474] | Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12237474 12237474] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kendall, D | + | [[Category: Kendall, D A.]] |
| - | [[Category: McMurry, J | + | [[Category: McMurry, J L.]] |
| - | [[Category: Mierke, D | + | [[Category: Mierke, D F.]] |
[[Category: Miller, A.]] | [[Category: Miller, A.]] | ||
| - | [[Category: Ulfers, A | + | [[Category: Ulfers, A L.]] |
[[Category: Wang, L.]] | [[Category: Wang, L.]] | ||
[[Category: alpha domain of g protein i]] | [[Category: alpha domain of g protein i]] | ||
| Line 24: | Line 23: | ||
[[Category: transferred noes]] | [[Category: transferred noes]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:57 2008'' |
Revision as of 11:48, 21 February 2008
|
IC3 of CB1 (L431A,A432L) Bound to G(alpha)i
Overview
The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.
About this Structure
1LVR is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474
Page seeded by OCA on Thu Feb 21 13:48:57 2008
