1ly2
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Human complement receptor type 2 (CD21) is the cellular receptor for | + | Human complement receptor type 2 (CD21) is the cellular receptor for Epstein-Barr virus (EBV), a human tumor virus. The N-terminal two short consensus repeats (SCR1-SCR2) of the receptor interact with the EBV glycoprotein gp350/220 and also with the natural CD21 ligand C3d. Here we present the crystal structure of the CD21 SCR1-SCR2 fragment in the absence of ligand and demonstrate that it is able to bind EBV. Based on a functional analysis of wild-type and mutant CD21 and molecular modeling, we identify a likely region for EBV attachment and demonstrate that this region is not involved in the interaction with C3d. A comparison with the previously determined structure of CD21 SCR1-SCR2 in complex with C3d shows that, in both cases, CD21 assumes compact V-shaped conformations. However, our analysis reveals a surprising degree of flexibility at the SCR1-SCR2 interface, suggesting interactions between the two domains are not specific. We present evidence that the V-shaped conformation is induced by deglycosylation of the protein, and that physiologic glycosylation of CD21 would result in a more extended conformation, perhaps with additional epitopes for C3d binding. |
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Fingeroth, J | + | [[Category: Fingeroth, J D.]] |
| - | [[Category: Prota, A | + | [[Category: Prota, A E.]] |
| - | [[Category: Sage, D | + | [[Category: Sage, D R.]] |
[[Category: Stehle, T.]] | [[Category: Stehle, T.]] | ||
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: complement receptor; epstein barr virus; regulator of complement activation; short consensus repeat; viral receptor; complement control protein]] | [[Category: complement receptor; epstein barr virus; regulator of complement activation; short consensus repeat; viral receptor; complement control protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:30 2008'' |
Revision as of 11:49, 21 February 2008
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Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)
Contents |
Overview
Human complement receptor type 2 (CD21) is the cellular receptor for Epstein-Barr virus (EBV), a human tumor virus. The N-terminal two short consensus repeats (SCR1-SCR2) of the receptor interact with the EBV glycoprotein gp350/220 and also with the natural CD21 ligand C3d. Here we present the crystal structure of the CD21 SCR1-SCR2 fragment in the absence of ligand and demonstrate that it is able to bind EBV. Based on a functional analysis of wild-type and mutant CD21 and molecular modeling, we identify a likely region for EBV attachment and demonstrate that this region is not involved in the interaction with C3d. A comparison with the previously determined structure of CD21 SCR1-SCR2 in complex with C3d shows that, in both cases, CD21 assumes compact V-shaped conformations. However, our analysis reveals a surprising degree of flexibility at the SCR1-SCR2 interface, suggesting interactions between the two domains are not specific. We present evidence that the V-shaped conformation is induced by deglycosylation of the protein, and that physiologic glycosylation of CD21 would result in a more extended conformation, perhaps with additional epitopes for C3d binding.
Disease
Known diseases associated with this structure: Systemic lupus erythematosus, susceptibility to, 9 OMIM:[120650]
About this Structure
1LY2 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of human CD21: Implications for Epstein-Barr virus and C3d binding., Prota AE, Sage DR, Stehle T, Fingeroth JD, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10641-6. Epub 2002 Jul 16. PMID:12122212
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