1lz0
From Proteopedia
(New page: 200px<br /> <applet load="1lz0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lz0, resolution 1.8Å" /> '''Glycosyltransferase ...) |
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| - | [[Image:1lz0.gif|left|200px]]<br /> | + | [[Image:1lz0.gif|left|200px]]<br /><applet load="1lz0" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lz0" size=" | + | |
caption="1lz0, resolution 1.8Å" /> | caption="1lz0, resolution 1.8Å" /> | ||
'''Glycosyltransferase A'''<br /> | '''Glycosyltransferase A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The human ABO(H) blood group antigens are produced by specific | + | The human ABO(H) blood group antigens are produced by specific glycosyltransferase enzymes. An N-acetylgalactosaminyltransferase (GTA) uses a UDP-GalNAc donor to convert the H-antigen acceptor to the A antigen, whereas a galactosyltransferase (GTB) uses a UDP-galactose donor to convert the H-antigen acceptor to the B antigen. GTA and GTB differ only in the identity of four critical amino acid residues. Crystal structures at 1.8-1.32 A resolution of the GTA and GTB enzymes both free and in complex with disaccharide H-antigen acceptor and UDP reveal the basis for donor and acceptor specificity and show that only two of the critical amino acid residues are positioned to contact donor or acceptor substrates. Given the need for stringent stereo- and regioselectivity in this biosynthesis, these structures further demonstrate that the ability of the two enzymes to distinguish between the A and B donors is largely determined by a single amino acid residue. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glycoprotein-fucosylgalactoside_alpha-N-acetylgalactosaminyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.40 2.4.1.40] Full crystallographic information is available from [http:// | + | 1LZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glycoprotein-fucosylgalactoside_alpha-N-acetylgalactosaminyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.40 2.4.1.40] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZ0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Borisova, S | + | [[Category: Borisova, S N.]] |
| - | [[Category: Evans, S | + | [[Category: Evans, S V.]] |
| - | [[Category: Marcus, S | + | [[Category: Marcus, S L.]] |
| - | [[Category: Palcic, M | + | [[Category: Palcic, M M.]] |
| - | [[Category: Patenaude, S | + | [[Category: Patenaude, S I.]] |
| - | [[Category: Seto, N | + | [[Category: Seto, N O.L.]] |
[[Category: Szpacenko, A.]] | [[Category: Szpacenko, A.]] | ||
[[Category: HG]] | [[Category: HG]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:45 2008'' |
Revision as of 11:49, 21 February 2008
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Glycosyltransferase A
Contents |
Overview
The human ABO(H) blood group antigens are produced by specific glycosyltransferase enzymes. An N-acetylgalactosaminyltransferase (GTA) uses a UDP-GalNAc donor to convert the H-antigen acceptor to the A antigen, whereas a galactosyltransferase (GTB) uses a UDP-galactose donor to convert the H-antigen acceptor to the B antigen. GTA and GTB differ only in the identity of four critical amino acid residues. Crystal structures at 1.8-1.32 A resolution of the GTA and GTB enzymes both free and in complex with disaccharide H-antigen acceptor and UDP reveal the basis for donor and acceptor specificity and show that only two of the critical amino acid residues are positioned to contact donor or acceptor substrates. Given the need for stringent stereo- and regioselectivity in this biosynthesis, these structures further demonstrate that the ability of the two enzymes to distinguish between the A and B donors is largely determined by a single amino acid residue.
Disease
Known disease associated with this structure: Blood group, ABO system OMIM:[110300]
About this Structure
1LZ0 is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, with EC number 2.4.1.40 Full crystallographic information is available from OCA.
Reference
The structural basis for specificity in human ABO(H) blood group biosynthesis., Patenaude SI, Seto NO, Borisova SN, Szpacenko A, Marcus SL, Palcic MM, Evans SV, Nat Struct Biol. 2002 Sep;9(9):685-90. PMID:12198488
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