1lz8
From Proteopedia
(New page: 200px<br /><applet load="1lz8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lz8, resolution 1.53Å" /> '''LYSOZYME PHASED ON A...) |
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| - | [[Image:1lz8.jpg|left|200px]]<br /><applet load="1lz8" size=" | + | [[Image:1lz8.jpg|left|200px]]<br /><applet load="1lz8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lz8, resolution 1.53Å" /> | caption="1lz8, resolution 1.53Å" /> | ||
'''LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES'''<br /> | '''LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A general method for solving the phase problem from native crystals of | + | A general method for solving the phase problem from native crystals of macromolecules has long eluded structural biology. For well diffracting crystals this goal can now be achieved, as is shown here, thanks to modern data collection techniques and new statistical phasing algorithms. Using solely a native crystal of tetragonal hen egg-white lysozyme, a protein of 14 kDa molecular mass, it was possible to detect the positions of the ten sulfur and seven chlorine atoms from their anomalous signal, and proceed from there to obtain an electron-density map of very high quality. |
==About this Structure== | ==About this Structure== | ||
| - | 1LZ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CL and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1LZ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZ8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dauter, M.]] | [[Category: Dauter, M.]] | ||
[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
| - | [[Category: Fortelle, E | + | [[Category: Fortelle, E De La.]] |
| - | [[Category: Sheldrick, G | + | [[Category: Sheldrick, G M.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: o-glycosyl]] | [[Category: o-glycosyl]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:52 2008'' |
Revision as of 11:49, 21 February 2008
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LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES
Overview
A general method for solving the phase problem from native crystals of macromolecules has long eluded structural biology. For well diffracting crystals this goal can now be achieved, as is shown here, thanks to modern data collection techniques and new statistical phasing algorithms. Using solely a native crystal of tetragonal hen egg-white lysozyme, a protein of 14 kDa molecular mass, it was possible to detect the positions of the ten sulfur and seven chlorine atoms from their anomalous signal, and proceed from there to obtain an electron-density map of very high quality.
About this Structure
1LZ8 is a Single protein structure of sequence from Gallus gallus with and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Can anomalous signal of sulfur become a tool for solving protein crystal structures?, Dauter Z, Dauter M, de La Fortelle E, Bricogne G, Sheldrick GM, J Mol Biol. 1999 May 28;289(1):83-92. PMID:10339407
Page seeded by OCA on Thu Feb 21 13:49:52 2008
Categories: Gallus gallus | Lysozyme | Single protein | Bricogne, G. | Dauter, M. | Dauter, Z. | Fortelle, E De La. | Sheldrick, G M. | CL | NA | Glycosidase | Hydrolase | O-glycosyl
