1m0g
From Proteopedia
(New page: 200px<br /><applet load="1m0g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m0g" /> '''Solution structure of the alpha domain of mt...) |
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| - | [[Image:1m0g.jpg|left|200px]]<br /><applet load="1m0g" size=" | + | [[Image:1m0g.jpg|left|200px]]<br /><applet load="1m0g" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of the alpha domain of mt_nc'''<br /> | '''Solution structure of the alpha domain of mt_nc'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish | + | The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins. |
==About this Structure== | ==About this Structure== | ||
| - | 1M0G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Notothenia_coriiceps Notothenia coriiceps] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1M0G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Notothenia_coriiceps Notothenia coriiceps] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Carginale, V.]] | [[Category: Carginale, V.]] | ||
[[Category: Crescenzi, O.]] | [[Category: Crescenzi, O.]] | ||
| - | [[Category: Maro, D | + | [[Category: Maro, D Di.]] |
[[Category: Parisi, E.]] | [[Category: Parisi, E.]] | ||
[[Category: Spadaccini, R.]] | [[Category: Spadaccini, R.]] | ||
| - | [[Category: Temussi, P | + | [[Category: Temussi, P A.]] |
[[Category: CD]] | [[Category: CD]] | ||
[[Category: cadmium-thiolate cluster]] | [[Category: cadmium-thiolate cluster]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:16 2008'' |
Revision as of 11:50, 21 February 2008
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Solution structure of the alpha domain of mt_nc
Overview
The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins.
About this Structure
1M0G is a Single protein structure of sequence from Notothenia coriiceps with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of MT_nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps., Capasso C, Carginale V, Crescenzi O, Di Maro D, Parisi E, Spadaccini R, Temussi PA, Structure. 2003 Apr;11(4):435-43. PMID:12679021
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