This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1m0j
From Proteopedia
(New page: 200px<br /><applet load="1m0j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m0j" /> '''solution structure of the beta domain of mt_...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1m0j.jpg|left|200px]]<br /><applet load="1m0j" size=" | + | [[Image:1m0j.jpg|left|200px]]<br /><applet load="1m0j" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1m0j" /> | caption="1m0j" /> | ||
'''solution structure of the beta domain of mt_nc'''<br /> | '''solution structure of the beta domain of mt_nc'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish | + | The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins. |
==About this Structure== | ==About this Structure== | ||
| - | 1M0J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Notothenia_coriiceps Notothenia coriiceps] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1M0J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Notothenia_coriiceps Notothenia coriiceps] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M0J OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Carginale, V.]] | [[Category: Carginale, V.]] | ||
[[Category: Crescenzi, O.]] | [[Category: Crescenzi, O.]] | ||
| - | [[Category: Maro, D | + | [[Category: Maro, D Di.]] |
[[Category: Parisi, E.]] | [[Category: Parisi, E.]] | ||
[[Category: Spadaccini, R.]] | [[Category: Spadaccini, R.]] | ||
| - | [[Category: Temussi, P | + | [[Category: Temussi, P A.]] |
[[Category: CD]] | [[Category: CD]] | ||
[[Category: cadmium thiolate-cluster]] | [[Category: cadmium thiolate-cluster]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:16 2008'' |
Revision as of 11:50, 21 February 2008
|
solution structure of the beta domain of mt_nc
Overview
The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins.
About this Structure
1M0J is a Single protein structure of sequence from Notothenia coriiceps with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of MT_nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps., Capasso C, Carginale V, Crescenzi O, Di Maro D, Parisi E, Spadaccini R, Temussi PA, Structure. 2003 Apr;11(4):435-43. PMID:12679021
Page seeded by OCA on Thu Feb 21 13:50:16 2008
