1m1c
From Proteopedia
(New page: 200px<br /><applet load="1m1c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m1c, resolution 3.5Å" /> '''Structure of the L-A ...) |
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| - | [[Image:1m1c.jpg|left|200px]]<br /><applet load="1m1c" size=" | + | [[Image:1m1c.jpg|left|200px]]<br /><applet load="1m1c" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1m1c, resolution 3.5Å" /> | caption="1m1c, resolution 3.5Å" /> | ||
'''Structure of the L-A virus'''<br /> | '''Structure of the L-A virus'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the yeast L-A virus was determined by X-ray | + | The structure of the yeast L-A virus was determined by X-ray crystallography at 3.4 A resolution. The L-A dsRNA virus is 400 A in diameter and contains a single protein shell of 60 asymmetric dimers of the coat protein, a feature common among the inner protein shells of dsRNA viruses and probably related to their unique mode of transcription and replication. The two identical subunits in each dimer are in non-equivalent environments and show substantially different conformations in specific surface regions. The L-A virus decaps cellular mRNA to efficiently translate its own uncapped mRNA. Our structure reveals a trench at the active site of the decapping reaction and suggests a role for nearby residues in the reaction. |
==About this Structure== | ==About this Structure== | ||
| - | 1M1C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_virus_l-a Saccharomyces cerevisiae virus l-a]. Full crystallographic information is available from [http:// | + | 1M1C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_virus_l-a Saccharomyces cerevisiae virus l-a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M1C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Canady, M.]] | [[Category: Canady, M.]] | ||
| - | [[Category: Johnson, J | + | [[Category: Johnson, J E.]] |
[[Category: Naitow, H.]] | [[Category: Naitow, H.]] | ||
[[Category: Tang, J.]] | [[Category: Tang, J.]] | ||
| - | [[Category: Wickner, R | + | [[Category: Wickner, R B.]] |
[[Category: dsrna virus structure; rna-protein interaction; mrna decapping; l-a virus; quai-equivalence]] | [[Category: dsrna virus structure; rna-protein interaction; mrna decapping; l-a virus; quai-equivalence]] | ||
[[Category: icosahedral virus]] | [[Category: icosahedral virus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:27 2008'' |
Revision as of 11:50, 21 February 2008
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Structure of the L-A virus
Overview
The structure of the yeast L-A virus was determined by X-ray crystallography at 3.4 A resolution. The L-A dsRNA virus is 400 A in diameter and contains a single protein shell of 60 asymmetric dimers of the coat protein, a feature common among the inner protein shells of dsRNA viruses and probably related to their unique mode of transcription and replication. The two identical subunits in each dimer are in non-equivalent environments and show substantially different conformations in specific surface regions. The L-A virus decaps cellular mRNA to efficiently translate its own uncapped mRNA. Our structure reveals a trench at the active site of the decapping reaction and suggests a role for nearby residues in the reaction.
About this Structure
1M1C is a Single protein structure of sequence from Saccharomyces cerevisiae virus l-a. Full crystallographic information is available from OCA.
Reference
L-A virus at 3.4 A resolution reveals particle architecture and mRNA decapping mechanism., Naitow H, Tang J, Canady M, Wickner RB, Johnson JE, Nat Struct Biol. 2002 Oct;9(10):725-8. PMID:12244300
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