1m4z
From Proteopedia
(New page: 200px<br /><applet load="1m4z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m4z, resolution 2.2Å" /> '''Crystal structure of ...) |
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| - | [[Image:1m4z.jpg|left|200px]]<br /><applet load="1m4z" size=" | + | [[Image:1m4z.jpg|left|200px]]<br /><applet load="1m4z" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1m4z, resolution 2.2Å" /> | caption="1m4z, resolution 2.2Å" /> | ||
'''Crystal structure of the N-terminal BAH domain of Orc1p'''<br /> | '''Crystal structure of the N-terminal BAH domain of Orc1p'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The N-terminal domain of the largest subunit of the Saccharomyces | + | The N-terminal domain of the largest subunit of the Saccharomyces cerevisiae origin recognition complex (Orc1p) functions in transcriptional silencing and contains a bromo-adjacent homology (BAH) domain found in some chromatin-associated proteins including Sir3p. The 2.2 A crystal structure of the N-terminal domain of Orc1p revealed a BAH core and a non-conserved helical sub-domain. Mutational analyses demonstrated that the helical sub-domain was necessary and sufficient to bind Sir1p, and critical for targeting Sir1p primarily to the cis-acting E silencers at the HMR and HML silent chromatin domains. In the absence of the BAH domain, approximately 14-20% of cells in a population were silenced at the HML locus. Moreover, the distributions of the Sir2p, Sir3p and Sir4p proteins, while normal, were at levels lower than found in wild-type cells. Thus, in the absence of the Orc1p BAH domain, HML resembled silencing of genes adjacent to telomeres. These data are consistent with the view that the Orc1p-Sir1p interaction at the E silencers ensures stable inheritance of pre-established Sir2p, Sir3p and Sir4p complexes at the silent mating type loci. |
==About this Structure== | ==About this Structure== | ||
| - | 1M4Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1M4Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hayashi, M | + | [[Category: Hayashi, M K.]] |
[[Category: Merkel, O.]] | [[Category: Merkel, O.]] | ||
[[Category: Stillman, B.]] | [[Category: Stillman, B.]] | ||
| - | [[Category: Xu, R | + | [[Category: Xu, R M.]] |
[[Category: Zhang, Z.]] | [[Category: Zhang, Z.]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: transcriptional silencing]] | [[Category: transcriptional silencing]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:33 2008'' |
Revision as of 11:51, 21 February 2008
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Crystal structure of the N-terminal BAH domain of Orc1p
Overview
The N-terminal domain of the largest subunit of the Saccharomyces cerevisiae origin recognition complex (Orc1p) functions in transcriptional silencing and contains a bromo-adjacent homology (BAH) domain found in some chromatin-associated proteins including Sir3p. The 2.2 A crystal structure of the N-terminal domain of Orc1p revealed a BAH core and a non-conserved helical sub-domain. Mutational analyses demonstrated that the helical sub-domain was necessary and sufficient to bind Sir1p, and critical for targeting Sir1p primarily to the cis-acting E silencers at the HMR and HML silent chromatin domains. In the absence of the BAH domain, approximately 14-20% of cells in a population were silenced at the HML locus. Moreover, the distributions of the Sir2p, Sir3p and Sir4p proteins, while normal, were at levels lower than found in wild-type cells. Thus, in the absence of the Orc1p BAH domain, HML resembled silencing of genes adjacent to telomeres. These data are consistent with the view that the Orc1p-Sir1p interaction at the E silencers ensures stable inheritance of pre-established Sir2p, Sir3p and Sir4p complexes at the silent mating type loci.
About this Structure
1M4Z is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Structure and function of the BAH-containing domain of Orc1p in epigenetic silencing., Zhang Z, Hayashi MK, Merkel O, Stillman B, Xu RM, EMBO J. 2002 Sep 2;21(17):4600-11. PMID:12198162
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