1m5n
From Proteopedia
(New page: 200px<br /> <applet load="1m5n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5n, resolution 2.90Å" /> '''Crystal structure o...) |
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| - | [[Image:1m5n.gif|left|200px]]<br /> | + | [[Image:1m5n.gif|left|200px]]<br /><applet load="1m5n" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1m5n" size=" | + | |
caption="1m5n, resolution 2.90Å" /> | caption="1m5n, resolution 2.90Å" /> | ||
'''Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP'''<br /> | '''Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nuclear import of proteins containing a classical nuclear localization | + | Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importin beta. We solved the crystal structure of a fragment of importin beta-1 (1-485) bound to the nonclassical NLS of PTHrP. The structure reveals a second extended cargo binding site on importin beta distinct from the IBB domain binding site. Using a permeabilized cell import assay we demonstrate that importin beta (1-485) can import PTHrP-coupled cargo in a Ran-dependent manner. We propose that this region contains a prototypical nuclear import receptor domain, which could have evolved into the modern importin beta superfamily. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1M5N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1M5N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cingolani, G.]] | [[Category: Cingolani, G.]] | ||
[[Category: Gerace, L.]] | [[Category: Gerace, L.]] | ||
| - | [[Category: Gillespie, M | + | [[Category: Gillespie, M T.]] |
[[Category: all helical protein]] | [[Category: all helical protein]] | ||
[[Category: heat repeats]] | [[Category: heat repeats]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:45 2008'' |
Revision as of 11:51, 21 February 2008
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Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP
Contents |
Overview
Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importin beta. We solved the crystal structure of a fragment of importin beta-1 (1-485) bound to the nonclassical NLS of PTHrP. The structure reveals a second extended cargo binding site on importin beta distinct from the IBB domain binding site. Using a permeabilized cell import assay we demonstrate that importin beta (1-485) can import PTHrP-coupled cargo in a Ran-dependent manner. We propose that this region contains a prototypical nuclear import receptor domain, which could have evolved into the modern importin beta superfamily.
Disease
Known disease associated with this structure: Humoral hypercalcemia of malignancy (1) OMIM:[168470]
About this Structure
1M5N is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta., Cingolani G, Bednenko J, Gillespie MT, Gerace L, Mol Cell. 2002 Dec;10(6):1345-53. PMID:12504010
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