1m5y

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(New page: 200px<br /><applet load="1m5y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5y, resolution 3.00&Aring;" /> '''Crystallographic Str...)
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'''Crystallographic Structure of SurA, a Molecular Chaperone that Facilitates Outer Membrane Porin Folding'''<br />
'''Crystallographic Structure of SurA, a Molecular Chaperone that Facilitates Outer Membrane Porin Folding'''<br />
==Overview==
==Overview==
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The SurA protein facilitates correct folding of outer membrane proteins in, gram-negative bacteria. The sequence of Escherichia coli SurA presents, four segments, two of which are peptidyl-prolyl isomerases (PPIases); the, crystal structure reveals an asymmetric dumbbell, in which the, amino-terminal, carboxy-terminal, and first PPIase segments of the, sequence form a core structural module, and the second PPIase segment is a, satellite domain tethered approximately 30 A from this module. The core, module, which is implicated in membrane protein folding, has a novel fold, that includes an extended crevice. Crystal contacts show that peptides, bind within the crevice, suggesting a model for chaperone activity whereby, segments of polypeptide may be repetitively sequestered and released, during the membrane protein-folding process.
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The SurA protein facilitates correct folding of outer membrane proteins in gram-negative bacteria. The sequence of Escherichia coli SurA presents four segments, two of which are peptidyl-prolyl isomerases (PPIases); the crystal structure reveals an asymmetric dumbbell, in which the amino-terminal, carboxy-terminal, and first PPIase segments of the sequence form a core structural module, and the second PPIase segment is a satellite domain tethered approximately 30 A from this module. The core module, which is implicated in membrane protein folding, has a novel fold that includes an extended crevice. Crystal contacts show that peptides bind within the crevice, suggesting a model for chaperone activity whereby segments of polypeptide may be repetitively sequestered and released during the membrane protein-folding process.
==About this Structure==
==About this Structure==
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1M5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M5Y OCA].
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1M5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5Y OCA].
==Reference==
==Reference==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Bitto, E.]]
[[Category: Bitto, E.]]
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[[Category: McKay, D.B.]]
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[[Category: McKay, D B.]]
[[Category: crystal structure]]
[[Category: crystal structure]]
[[Category: gram negative bacteria]]
[[Category: gram negative bacteria]]
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[[Category: survival protein a]]
[[Category: survival protein a]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:10:17 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:50 2008''

Revision as of 11:51, 21 February 2008


1m5y, resolution 3.00Å

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Crystallographic Structure of SurA, a Molecular Chaperone that Facilitates Outer Membrane Porin Folding

Overview

The SurA protein facilitates correct folding of outer membrane proteins in gram-negative bacteria. The sequence of Escherichia coli SurA presents four segments, two of which are peptidyl-prolyl isomerases (PPIases); the crystal structure reveals an asymmetric dumbbell, in which the amino-terminal, carboxy-terminal, and first PPIase segments of the sequence form a core structural module, and the second PPIase segment is a satellite domain tethered approximately 30 A from this module. The core module, which is implicated in membrane protein folding, has a novel fold that includes an extended crevice. Crystal contacts show that peptides bind within the crevice, suggesting a model for chaperone activity whereby segments of polypeptide may be repetitively sequestered and released during the membrane protein-folding process.

About this Structure

1M5Y is a Single protein structure of sequence from Escherichia coli. Active as Peptidylprolyl isomerase, with EC number 5.2.1.8 Full crystallographic information is available from OCA.

Reference

Crystallographic structure of SurA, a molecular chaperone that facilitates folding of outer membrane porins., Bitto E, McKay DB, Structure. 2002 Nov;10(11):1489-98. PMID:12429090

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