1m5w
From Proteopedia
(New page: 200px<br /><applet load="1m5w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5w, resolution 1.96Å" /> '''1.96 A Crystal Struc...) |
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| - | [[Image:1m5w.gif|left|200px]]<br /><applet load="1m5w" size=" | + | [[Image:1m5w.gif|left|200px]]<br /><applet load="1m5w" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1m5w, resolution 1.96Å" /> | caption="1m5w, resolution 1.96Å" /> | ||
'''1.96 A Crystal Structure of Pyridoxine 5'-Phosphate Synthase in Complex with 1-deoxy-D-xylulose phosphate'''<br /> | '''1.96 A Crystal Structure of Pyridoxine 5'-Phosphate Synthase in Complex with 1-deoxy-D-xylulose phosphate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report the 1.96 A crystal structure of pyridoxine 5'-phosphate synthase | + | We report the 1.96 A crystal structure of pyridoxine 5'-phosphate synthase (PdxJ) in complex with 1-deoxy-D-xylulose phosphate (dXP). The octameric enzyme possesses eight distinct binding sites, and three different binding states are observed. The observation of these three states supports a mechanism in which precise conformational changes of a peptide loop and groups of active site residues modulate binding and specificity. The differences in protein conformation when one or two substrates are bound can be correlated with a condensation mechanism that leads productively to the formation of pyridoxine 5'-phosphate (PNP). "Snapshots" of the progression from the apo form to a singly occupied "transitional binding" state and, subsequently, to a fully occupied, reactive state are revealed and indicate how the enzyme structure can be related to a plausible catalytic mechanism and, moreover, to favorable energetics of reaction. |
==About this Structure== | ==About this Structure== | ||
| - | 1M5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with DXP and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1M5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=DXP:'>DXP</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cane, D | + | [[Category: Cane, D E.]] |
[[Category: Du, S.]] | [[Category: Du, S.]] | ||
[[Category: Pohl, E.]] | [[Category: Pohl, E.]] | ||
| - | [[Category: Yeh, J | + | [[Category: Yeh, J I.]] |
[[Category: DXP]] | [[Category: DXP]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:48 2008'' |
Revision as of 11:51, 21 February 2008
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1.96 A Crystal Structure of Pyridoxine 5'-Phosphate Synthase in Complex with 1-deoxy-D-xylulose phosphate
Overview
We report the 1.96 A crystal structure of pyridoxine 5'-phosphate synthase (PdxJ) in complex with 1-deoxy-D-xylulose phosphate (dXP). The octameric enzyme possesses eight distinct binding sites, and three different binding states are observed. The observation of these three states supports a mechanism in which precise conformational changes of a peptide loop and groups of active site residues modulate binding and specificity. The differences in protein conformation when one or two substrates are bound can be correlated with a condensation mechanism that leads productively to the formation of pyridoxine 5'-phosphate (PNP). "Snapshots" of the progression from the apo form to a singly occupied "transitional binding" state and, subsequently, to a fully occupied, reactive state are revealed and indicate how the enzyme structure can be related to a plausible catalytic mechanism and, moreover, to favorable energetics of reaction.
About this Structure
1M5W is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
Reference
Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate., Yeh JI, Du S, Pohl E, Cane DE, Biochemistry. 2002 Oct 1;41(39):11649-57. PMID:12269807
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