1m8y
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Puf proteins are developmental regulators that control mRNA stability and | + | Puf proteins are developmental regulators that control mRNA stability and translation by binding sequences in the 3' untranslated regions of their target mRNAs. We have determined the structure of the RNA binding domain of the human Puf protein, Pumilio1, bound to a high-affinity RNA ligand. The RNA binds the concave surface of the molecule, where each of the protein's eight repeats makes contacts with a different RNA base via three amino acid side chains at conserved positions. We have mutated these three side chains in one repeat, thereby altering the sequence specificity of Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is achieved using multiple copies of a simple repeated motif. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hall, T | + | [[Category: Hall, T M.T.]] |
[[Category: McLachlan, J.]] | [[Category: McLachlan, J.]] | ||
[[Category: Wang, X.]] | [[Category: Wang, X.]] | ||
| - | [[Category: Zamore, P | + | [[Category: Zamore, P D.]] |
[[Category: nanos response element]] | [[Category: nanos response element]] | ||
[[Category: puf domain]] | [[Category: puf domain]] | ||
[[Category: pumilio-homology domain]] | [[Category: pumilio-homology domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:52:46 2008'' |
Revision as of 11:52, 21 February 2008
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CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE2-10 RNA
Overview
Puf proteins are developmental regulators that control mRNA stability and translation by binding sequences in the 3' untranslated regions of their target mRNAs. We have determined the structure of the RNA binding domain of the human Puf protein, Pumilio1, bound to a high-affinity RNA ligand. The RNA binds the concave surface of the molecule, where each of the protein's eight repeats makes contacts with a different RNA base via three amino acid side chains at conserved positions. We have mutated these three side chains in one repeat, thereby altering the sequence specificity of Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is achieved using multiple copies of a simple repeated motif.
About this Structure
1M8Y is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Modular recognition of RNA by a human pumilio-homology domain., Wang X, McLachlan J, Zamore PD, Hall TM, Cell. 2002 Aug 23;110(4):501-12. PMID:12202039
Page seeded by OCA on Thu Feb 21 13:52:46 2008
