1ma8
From Proteopedia
(New page: 200px<br /><applet load="1ma8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ma8, resolution 1.3Å" /> '''A-DNA decamer GCGTA(U...) |
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| - | [[Image:1ma8.gif|left|200px]]<br /><applet load="1ma8" size=" | + | [[Image:1ma8.gif|left|200px]]<br /><applet load="1ma8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ma8, resolution 1.3Å" /> | caption="1ma8, resolution 1.3Å" /> | ||
'''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine'''<br /> | '''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Selenium was incorporated into an oligodeoxynucleotide in the form of | + | Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines. |
==About this Structure== | ==About this Structure== | ||
| - | 1MA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1MA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Tereshko, V.]] | [[Category: Tereshko, V.]] | ||
[[Category: Wawrzak, Z.]] | [[Category: Wawrzak, Z.]] | ||
| - | [[Category: Wilds, C | + | [[Category: Wilds, C J.]] |
[[Category: 2'-methylseleno-uridine]] | [[Category: 2'-methylseleno-uridine]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:09 2008'' |
Revision as of 11:53, 21 February 2008
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A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine
Overview
Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
About this Structure
1MA8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:12458077
Page seeded by OCA on Thu Feb 21 13:53:09 2008
