1mbm
From Proteopedia
(New page: 200px<br /><applet load="1mbm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mbm, resolution 2.00Å" /> '''NSP4 proteinase from...) |
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| - | [[Image:1mbm.jpg|left|200px]]<br /><applet load="1mbm" size=" | + | [[Image:1mbm.jpg|left|200px]]<br /><applet load="1mbm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mbm, resolution 2.00Å" /> | caption="1mbm, resolution 2.00Å" /> | ||
'''NSP4 proteinase from Equine Arteritis Virus'''<br /> | '''NSP4 proteinase from Equine Arteritis Virus'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Arteriviruses are enveloped, positive-stranded RNA viruses and include | + | Arteriviruses are enveloped, positive-stranded RNA viruses and include pathogens of major economic concern to the swine- and horse-breeding industries. The arterivirus replicase gene encodes two large precursor polyproteins that are processed by the viral main proteinase nonstructural protein 4 (nsp4). The three-dimensional structure of the 21-kDa nsp4 from the arterivirus prototype equine arteritis virus has been determined to 2.0 A resolution. Nsp4 adopts the smallest known chymotrypsin-like fold with a canonical catalytic triad of Ser-120, His-39, and Asp-65, as well as a novel alpha/beta C-terminal extension domain that may play a role in mediating protein-protein interactions. In different copies of nsp4 in the asymmetric unit, the oxyanion hole adopts either a collapsed inactive conformation or the standard active conformation, which may be a novel way of regulating proteolytic activity. |
==About this Structure== | ==About this Structure== | ||
| - | 1MBM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equine_arteritis_virus Equine arteritis virus]. Full crystallographic information is available from [http:// | + | 1MBM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equine_arteritis_virus Equine arteritis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Equine arteritis virus]] | [[Category: Equine arteritis virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Aken, D | + | [[Category: Aken, D van.]] |
| - | [[Category: Barrette-Ng, I | + | [[Category: Barrette-Ng, I H.]] |
| - | [[Category: Cherney, M | + | [[Category: Cherney, M M.]] |
[[Category: Garen, C.]] | [[Category: Garen, C.]] | ||
| - | [[Category: Gorbalenya, A | + | [[Category: Gorbalenya, A E.]] |
| - | [[Category: James, M | + | [[Category: James, M N.G.]] |
[[Category: Kolodenko, Y.]] | [[Category: Kolodenko, Y.]] | ||
| - | [[Category: Mark, B | + | [[Category: Mark, B L.]] |
| - | [[Category: Ng, K | + | [[Category: Ng, K K.S.]] |
| - | [[Category: Snijder, E | + | [[Category: Snijder, E J.]] |
[[Category: chymotrypsin-like proteinase]] | [[Category: chymotrypsin-like proteinase]] | ||
[[Category: collapsed oxyanion hole]] | [[Category: collapsed oxyanion hole]] | ||
[[Category: serine proteinase]] | [[Category: serine proteinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:39 2008'' |
Revision as of 11:53, 21 February 2008
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NSP4 proteinase from Equine Arteritis Virus
Overview
Arteriviruses are enveloped, positive-stranded RNA viruses and include pathogens of major economic concern to the swine- and horse-breeding industries. The arterivirus replicase gene encodes two large precursor polyproteins that are processed by the viral main proteinase nonstructural protein 4 (nsp4). The three-dimensional structure of the 21-kDa nsp4 from the arterivirus prototype equine arteritis virus has been determined to 2.0 A resolution. Nsp4 adopts the smallest known chymotrypsin-like fold with a canonical catalytic triad of Ser-120, His-39, and Asp-65, as well as a novel alpha/beta C-terminal extension domain that may play a role in mediating protein-protein interactions. In different copies of nsp4 in the asymmetric unit, the oxyanion hole adopts either a collapsed inactive conformation or the standard active conformation, which may be a novel way of regulating proteolytic activity.
About this Structure
1MBM is a Single protein structure of sequence from Equine arteritis virus. Full crystallographic information is available from OCA.
Reference
Structure of arterivirus nsp4. The smallest chymotrypsin-like proteinase with an alpha/beta C-terminal extension and alternate conformations of the oxyanion hole., Barrette-Ng IH, Ng KK, Mark BL, Van Aken D, Cherney MM, Garen C, Kolodenko Y, Gorbalenya AE, Snijder EJ, James MN, J Biol Chem. 2002 Oct 18;277(42):39960-6. Epub 2002 Aug 5. PMID:12163505
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