1mc7
From Proteopedia
(New page: 200px<br /><applet load="1mc7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mc7" /> '''Solution Structure of mDvl1 PDZ domain'''<br...) |
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| - | [[Image:1mc7.jpg|left|200px]]<br /><applet load="1mc7" size=" | + | [[Image:1mc7.jpg|left|200px]]<br /><applet load="1mc7" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution Structure of mDvl1 PDZ domain'''<br /> | '''Solution Structure of mDvl1 PDZ domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The cytoplasmic protein Dishevelled (Dvl) and the associated | + | The cytoplasmic protein Dishevelled (Dvl) and the associated membrane-bound receptor Frizzled (Fz) are essential in canonical and noncanonical Wnt signaling pathways. However, the molecular mechanisms underlying this signaling are not well understood. By using NMR spectroscopy, we determined that an internal sequence of Fz binds to the conventional peptide binding site in the PDZ domain of Dvl; this type of site typically binds to C-terminal binding motifs. The C-terminal region of the Dvl inhibitor Dapper (Dpr) and Frodo bound to the same site. In Xenopus, Dvl binding peptides of Fz and Dpr/Frodo inhibited canonical Wnt signaling and blocked Wnt-induced secondary axis formation in a dose-dependent manner, but did not block noncanonical Wnt signaling mediated by the DEP domain. Together, our results identify a missing molecular connection within the Wnt pathway. Differences in the binding affinity of the Dvl PDZ domain and its binding partners may be important in regulating signal transduction by Dvl. |
==About this Structure== | ==About this Structure== | ||
| - | 1MC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1MC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bourdelas, A.]] | [[Category: Bourdelas, A.]] | ||
[[Category: Shao, Y.]] | [[Category: Shao, Y.]] | ||
| - | [[Category: Shi, D | + | [[Category: Shi, D L.]] |
| - | [[Category: Wong, H | + | [[Category: Wong, H C.]] |
[[Category: Wu, D.]] | [[Category: Wu, D.]] | ||
[[Category: Zheng, J.]] | [[Category: Zheng, J.]] | ||
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[[Category: wnt signaling]] | [[Category: wnt signaling]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:48 2008'' |
Revision as of 11:53, 21 February 2008
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Solution Structure of mDvl1 PDZ domain
Overview
The cytoplasmic protein Dishevelled (Dvl) and the associated membrane-bound receptor Frizzled (Fz) are essential in canonical and noncanonical Wnt signaling pathways. However, the molecular mechanisms underlying this signaling are not well understood. By using NMR spectroscopy, we determined that an internal sequence of Fz binds to the conventional peptide binding site in the PDZ domain of Dvl; this type of site typically binds to C-terminal binding motifs. The C-terminal region of the Dvl inhibitor Dapper (Dpr) and Frodo bound to the same site. In Xenopus, Dvl binding peptides of Fz and Dpr/Frodo inhibited canonical Wnt signaling and blocked Wnt-induced secondary axis formation in a dose-dependent manner, but did not block noncanonical Wnt signaling mediated by the DEP domain. Together, our results identify a missing molecular connection within the Wnt pathway. Differences in the binding affinity of the Dvl PDZ domain and its binding partners may be important in regulating signal transduction by Dvl.
About this Structure
1MC7 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled., Wong HC, Bourdelas A, Krauss A, Lee HJ, Shao Y, Wu D, Mlodzik M, Shi DL, Zheng J, Mol Cell. 2003 Nov;12(5):1251-60. PMID:14636582
Page seeded by OCA on Thu Feb 21 13:53:48 2008
Categories: Mus musculus | Single protein | Bourdelas, A. | Shao, Y. | Shi, D L. | Wong, H C. | Wu, D. | Zheng, J. | Pdz domain | Wnt signaling
