1mey
From Proteopedia
(New page: 200px<br /><applet load="1mey" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mey, resolution 2.200Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1mey.gif|left|200px]]<br /><applet load="1mey" size=" | + | [[Image:1mey.gif|left|200px]]<br /><applet load="1mey" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mey, resolution 2.200Å" /> | caption="1mey, resolution 2.200Å" /> | ||
'''CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA'''<br /> | '''CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Considerable recent effort has been devoted to the design and selection of | + | Considerable recent effort has been devoted to the design and selection of sequence-specific DNA binding proteins based on tandem arrays of Cys2His2 zinc finger domains. While the DNA binding properties of these designed proteins have been studied extensively, the structural basis for site-specific binding has not been examined experimentally. Here we report the crystal structure of a complex between a protein comprised of three consensus-sequence-based zinc finger domains and an oligonucleotide corresponding to a favourable DNA binding site. This structure reveals relatively simple modular interactions and structural adaptations that compensate for differences in contact residue side-chain lengths. |
==About this Structure== | ==About this Structure== | ||
| - | 1MEY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ZN and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1MEY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Berg, J | + | [[Category: Berg, J M.]] |
| - | [[Category: Kim, C | + | [[Category: Kim, C A.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:39 2008'' |
Revision as of 11:54, 21 February 2008
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CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA
Overview
Considerable recent effort has been devoted to the design and selection of sequence-specific DNA binding proteins based on tandem arrays of Cys2His2 zinc finger domains. While the DNA binding properties of these designed proteins have been studied extensively, the structural basis for site-specific binding has not been examined experimentally. Here we report the crystal structure of a complex between a protein comprised of three consensus-sequence-based zinc finger domains and an oligonucleotide corresponding to a favourable DNA binding site. This structure reveals relatively simple modular interactions and structural adaptations that compensate for differences in contact residue side-chain lengths.
About this Structure
1MEY is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
A 2.2 A resolution crystal structure of a designed zinc finger protein bound to DNA., Kim CA, Berg JM, Nat Struct Biol. 1996 Nov;3(11):940-5. PMID:8901872 [[Category: ]]
Page seeded by OCA on Thu Feb 21 13:54:39 2008
