1mhd
From Proteopedia
(New page: 200px<br /> <applet load="1mhd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mhd, resolution 2.8Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1mhd.gif|left|200px]]<br /> | + | [[Image:1mhd.gif|left|200px]]<br /><applet load="1mhd" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1mhd, resolution 2.8Å" /> | caption="1mhd, resolution 2.8Å" /> | ||
'''CRYSTAL STRUCTURE OF A SMAD MH1 DOMAIN BOUND TO DNA'''<br /> | '''CRYSTAL STRUCTURE OF A SMAD MH1 DOMAIN BOUND TO DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Smad family of proteins, which are frequently targeted by tumorigenic | + | The Smad family of proteins, which are frequently targeted by tumorigenic mutations in cancer, mediate TGF-beta signaling from cell membrane to nucleus. The crystal structure of a Smad3 MH1 domain bound to an optimal DNA sequence determined at 2.8 A resolution reveals a novel DNA-binding motif. In the crystals, base-specific DNA recognition is provided exclusively by a conserved 11-residue beta hairpin that is embedded in the major groove of DNA. A surface loop region, to which tumorigenic mutations map, has been identified as a functional surface important for Smad activity. This structure establishes a framework for understanding how Smad proteins may act in concert with other transcription factors in the regulation of TGF-beta-responsive genes. |
==About this Structure== | ==About this Structure== | ||
| - | 1MHD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1MHD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MHD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: smad3 mh1]] | [[Category: smad3 mh1]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:09 2008'' |
Revision as of 11:55, 21 February 2008
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CRYSTAL STRUCTURE OF A SMAD MH1 DOMAIN BOUND TO DNA
Overview
The Smad family of proteins, which are frequently targeted by tumorigenic mutations in cancer, mediate TGF-beta signaling from cell membrane to nucleus. The crystal structure of a Smad3 MH1 domain bound to an optimal DNA sequence determined at 2.8 A resolution reveals a novel DNA-binding motif. In the crystals, base-specific DNA recognition is provided exclusively by a conserved 11-residue beta hairpin that is embedded in the major groove of DNA. A surface loop region, to which tumorigenic mutations map, has been identified as a functional surface important for Smad activity. This structure establishes a framework for understanding how Smad proteins may act in concert with other transcription factors in the regulation of TGF-beta-responsive genes.
About this Structure
1MHD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a Smad MH1 domain bound to DNA: insights on DNA binding in TGF-beta signaling., Shi Y, Wang YF, Jayaraman L, Yang H, Massague J, Pavletich NP, Cell. 1998 Sep 4;94(5):585-94. PMID:9741623
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