1mi0
From Proteopedia
(New page: 200px<br /><applet load="1mi0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mi0, resolution 1.85Å" /> '''Crystal Structure of...) |
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| - | [[Image:1mi0.gif|left|200px]]<br /><applet load="1mi0" size=" | + | [[Image:1mi0.gif|left|200px]]<br /><applet load="1mi0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mi0, resolution 1.85Å" /> | caption="1mi0, resolution 1.85Å" /> | ||
'''Crystal Structure of the redesigned protein G variant NuG2'''<br /> | '''Crystal Structure of the redesigned protein G variant NuG2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We recently described two protein G variants (NuG1 and NuG2) with | + | We recently described two protein G variants (NuG1 and NuG2) with redesigned first hairpins that were almost twice as stable, folded 100-fold faster, and had a switched folding mechanism relative to the wild-type protein. To test the structural accuracy of our design algorithm and to provide insights to the dramatic changes in the kinetics and thermodynamics of folding, we have now determined the crystal structures of NuG1 and NuG2 to 1.8 A and 1.85 A, respectively. We find that they adopt hairpin structures that are closer to the computational models than to wild-type protein G; the RMSD of the NuG1 hairpin to the design model and the wild-type structure are 1.7 A and 5.1 A, respectively. The crystallographic B factor in the redesigned first hairpin of NuG1 is systematically higher than the second hairpin, suggesting that the redesigned region is somewhat less rigid. A second round of structure-based design yielded new variants of NuG1 and NuG2, which are further stabilized by 0.5 kcal/mole and 0.9 kcal/mole. |
==About this Structure== | ==About this Structure== | ||
| - | 1MI0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna]. Full crystallographic information is available from [http:// | + | 1MI0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kuhlman, B.]] | [[Category: Kuhlman, B.]] | ||
[[Category: Nauli, S.]] | [[Category: Nauli, S.]] | ||
| - | [[Category: Stenkamp, R | + | [[Category: Stenkamp, R E.]] |
| - | [[Category: Teller, D | + | [[Category: Teller, D C.]] |
| - | [[Category: Trong, I | + | [[Category: Trong, I Le.]] |
[[Category: alpha-beta protein]] | [[Category: alpha-beta protein]] | ||
[[Category: redesigned beta-hairpin]] | [[Category: redesigned beta-hairpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:26 2008'' |
Revision as of 11:55, 21 February 2008
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Crystal Structure of the redesigned protein G variant NuG2
Overview
We recently described two protein G variants (NuG1 and NuG2) with redesigned first hairpins that were almost twice as stable, folded 100-fold faster, and had a switched folding mechanism relative to the wild-type protein. To test the structural accuracy of our design algorithm and to provide insights to the dramatic changes in the kinetics and thermodynamics of folding, we have now determined the crystal structures of NuG1 and NuG2 to 1.8 A and 1.85 A, respectively. We find that they adopt hairpin structures that are closer to the computational models than to wild-type protein G; the RMSD of the NuG1 hairpin to the design model and the wild-type structure are 1.7 A and 5.1 A, respectively. The crystallographic B factor in the redesigned first hairpin of NuG1 is systematically higher than the second hairpin, suggesting that the redesigned region is somewhat less rigid. A second round of structure-based design yielded new variants of NuG1 and NuG2, which are further stabilized by 0.5 kcal/mole and 0.9 kcal/mole.
About this Structure
1MI0 is a Single protein structure of sequence from Finegoldia magna. Full crystallographic information is available from OCA.
Reference
Crystal structures and increased stabilization of the protein G variants with switched folding pathways NuG1 and NuG2., Nauli S, Kuhlman B, Le Trong I, Stenkamp RE, Teller D, Baker D, Protein Sci. 2002 Dec;11(12):2924-31. PMID:12441390
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