1mif
From Proteopedia
(New page: 200px<br /> <applet load="1mif" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mif, resolution 2.6Å" /> '''MACROPHAGE MIGRATION...) |
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| - | [[Image:1mif.gif|left|200px]]<br /> | + | [[Image:1mif.gif|left|200px]]<br /><applet load="1mif" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1mif" size=" | + | |
caption="1mif, resolution 2.6Å" /> | caption="1mif, resolution 2.6Å" /> | ||
'''MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)'''<br /> | '''MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Macrophage migration inhibitory factor (MIF) was the first cytokine to be | + | Macrophage migration inhibitory factor (MIF) was the first cytokine to be described, but for 30 years its role in the immune response remained enigmatic. In recent studies, MIF has been found to be a novel pituitary hormone and the first protein identified to be released from immune cells on glucocorticoid stimulation. Once secreted, MIF counterregulates the immunosuppressive effects of steroids and thus acts as a critical component of the immune system to control both local and systemic immune responses. We report herein the x-ray crystal structure of human MIF to 2.6 angstrom resolution. The protein is a trimer of identical subunits. Each monomer contains two antiparallel alpha-helices that pack against a four-stranded beta-sheet. The monomer has an additional two beta-strands that interact with the beta-sheets of adjacent subunits to form the interface between monomers. The three beta-sheets are arranged to form a barrel containing a solvent-accessible channel that runs through the center of the protein along a molecular 3-fold axis. Electrostatic potential maps reveal that the channel has a positive potential, suggesting that it binds negatively charged molecules. The elucidated structure for MIF is unique among cytokines or hormonal mediators, and suggests that this counterregulator of glucocorticoid action participates in novel ligand-receptor interactions. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1MIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Lolis, E.]] | [[Category: Lolis, E.]] | ||
| - | [[Category: Sun, H | + | [[Category: Sun, H W.]] |
[[Category: cytokine]] | [[Category: cytokine]] | ||
[[Category: glutathione binding protein]] | [[Category: glutathione binding protein]] | ||
[[Category: hormone]] | [[Category: hormone]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:28 2008'' |
Revision as of 11:55, 21 February 2008
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MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)
Contents |
Overview
Macrophage migration inhibitory factor (MIF) was the first cytokine to be described, but for 30 years its role in the immune response remained enigmatic. In recent studies, MIF has been found to be a novel pituitary hormone and the first protein identified to be released from immune cells on glucocorticoid stimulation. Once secreted, MIF counterregulates the immunosuppressive effects of steroids and thus acts as a critical component of the immune system to control both local and systemic immune responses. We report herein the x-ray crystal structure of human MIF to 2.6 angstrom resolution. The protein is a trimer of identical subunits. Each monomer contains two antiparallel alpha-helices that pack against a four-stranded beta-sheet. The monomer has an additional two beta-strands that interact with the beta-sheets of adjacent subunits to form the interface between monomers. The three beta-sheets are arranged to form a barrel containing a solvent-accessible channel that runs through the center of the protein along a molecular 3-fold axis. Electrostatic potential maps reveal that the channel has a positive potential, suggesting that it binds negatively charged molecules. The elucidated structure for MIF is unique among cytokines or hormonal mediators, and suggests that this counterregulator of glucocorticoid action participates in novel ligand-receptor interactions.
Disease
Known diseases associated with this structure: Persistent Mullerian duct syndrome, type I OMIM:[600957], Rheumatoid arthritis, systemic juvenile, susceptibility to OMIM:[153620]
About this Structure
1MIF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure at 2.6-A resolution of human macrophage migration inhibitory factor., Sun HW, Bernhagen J, Bucala R, Lolis E, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5191-6. PMID:8643551
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