1mij
From Proteopedia
(New page: 200px<br /><applet load="1mij" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mij, resolution 2.05Å" /> '''Crystal Structure of...) |
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| - | [[Image:1mij.gif|left|200px]]<br /><applet load="1mij" size=" | + | [[Image:1mij.gif|left|200px]]<br /><applet load="1mij" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mij, resolution 2.05Å" /> | caption="1mij, resolution 2.05Å" /> | ||
'''Crystal Structure of the Homeo-prospero Domain of D. melanogaster Prospero'''<br /> | '''Crystal Structure of the Homeo-prospero Domain of D. melanogaster Prospero'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Prospero transcription factor promotes neural differentiation in | + | The Prospero transcription factor promotes neural differentiation in Drosophila, and its activity is tightly regulated by modulating its subcellular localization. Prospero is exported from the nucleus of neural precursors but imported into the nucleus of daughter cells, which is necessary for their proper differentiation. Prospero has a highly divergent putative homeodomain adjacent to a conserved Prospero domain; both are required for sequence-specific DNA binding. Here we show that the structure of these two regions consists of a single structural unit (a homeo-prospero domain), in which the Prospero domain region is in position to contribute to DNA binding and also to mask a defined nuclear export signal that is within the putative homeodomain region. We propose that the homeo-prospero domain coordinately regulates Prospero nuclear localization and DNA binding specificity. |
==About this Structure== | ==About this Structure== | ||
| - | 1MIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1MIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Doe, C | + | [[Category: Doe, C Q.]] |
| - | [[Category: Matthews, B | + | [[Category: Matthews, B W.]] |
| - | [[Category: Ryter, J | + | [[Category: Ryter, J M.]] |
[[Category: 4-helix bundle]] | [[Category: 4-helix bundle]] | ||
[[Category: dna-binding domain]] | [[Category: dna-binding domain]] | ||
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[[Category: prospero domain]] | [[Category: prospero domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:32 2008'' |
Revision as of 11:55, 21 February 2008
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Crystal Structure of the Homeo-prospero Domain of D. melanogaster Prospero
Overview
The Prospero transcription factor promotes neural differentiation in Drosophila, and its activity is tightly regulated by modulating its subcellular localization. Prospero is exported from the nucleus of neural precursors but imported into the nucleus of daughter cells, which is necessary for their proper differentiation. Prospero has a highly divergent putative homeodomain adjacent to a conserved Prospero domain; both are required for sequence-specific DNA binding. Here we show that the structure of these two regions consists of a single structural unit (a homeo-prospero domain), in which the Prospero domain region is in position to contribute to DNA binding and also to mask a defined nuclear export signal that is within the putative homeodomain region. We propose that the homeo-prospero domain coordinately regulates Prospero nuclear localization and DNA binding specificity.
About this Structure
1MIJ is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of the DNA binding region of prospero reveals a novel homeo-prospero domain., Ryter JM, Doe CQ, Matthews BW, Structure. 2002 Nov;10(11):1541-9. PMID:12429095
Page seeded by OCA on Thu Feb 21 13:55:32 2008
