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1mj1
From Proteopedia
(New page: 200px<br /><applet load="1mj1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mj1" /> '''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GT...) |
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| - | [[Image:1mj1.gif|left|200px]]<br /><applet load="1mj1" size=" | + | [[Image:1mj1.gif|left|200px]]<br /><applet load="1mj1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mj1" /> | caption="1mj1" /> | ||
'''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME'''<br /> | '''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA | + | The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation. |
==About this Structure== | ==About this Structure== | ||
| - | 1MJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1MJ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJ1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Rodnina, M | + | [[Category: Rodnina, M V.]] |
[[Category: Stark, H.]] | [[Category: Stark, H.]] | ||
[[Category: Vanheel, M.]] | [[Category: Vanheel, M.]] | ||
| - | [[Category: Wieden, H | + | [[Category: Wieden, H J.]] |
[[Category: Wintermeyer, W.]] | [[Category: Wintermeyer, W.]] | ||
[[Category: Zemlin, F.]] | [[Category: Zemlin, F.]] | ||
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[[Category: low resolution model ternary complex]] | [[Category: low resolution model ternary complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:40 2008'' |
Revision as of 11:55, 21 February 2008
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FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME
Overview
The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation.
About this Structure
1MJ1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex., Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel M, Nat Struct Biol. 2002 Nov;9(11):849-54. PMID:12379845
Page seeded by OCA on Thu Feb 21 13:55:40 2008
