1mjg
From Proteopedia
(New page: 200px<br /><applet load="1mjg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mjg, resolution 2.2Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1mjg.gif|left|200px]]<br /><applet load="1mjg" size=" | + | [[Image:1mjg.gif|left|200px]]<br /><applet load="1mjg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mjg, resolution 2.2Å" /> | caption="1mjg, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)'''<br /> | '''CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A metallocofactor containing iron, sulfur, copper, and nickel has been | + | A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit. |
==About this Structure== | ==About this Structure== | ||
| - | 1MJG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica] with CU1, NI, ACT, SF4 and XCC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] Full crystallographic information is available from [http:// | + | 1MJG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica] with <scene name='pdbligand=CU1:'>CU1</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=XCC:'>XCC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Moorella thermoacetica]] | [[Category: Moorella thermoacetica]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Doukov, T | + | [[Category: Doukov, T I.]] |
| - | [[Category: Drennan, C | + | [[Category: Drennan, C L.]] |
| - | [[Category: Iverson, T | + | [[Category: Iverson, T M.]] |
| - | [[Category: Ragsdale, S | + | [[Category: Ragsdale, S W.]] |
[[Category: Seravalli, J.]] | [[Category: Seravalli, J.]] | ||
[[Category: ACT]] | [[Category: ACT]] | ||
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[[Category: wood-ljundahl pathway]] | [[Category: wood-ljundahl pathway]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:45 2008'' |
Revision as of 11:55, 21 February 2008
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CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)
Overview
A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit.
About this Structure
1MJG is a Protein complex structure of sequences from Moorella thermoacetica with , , , and as ligands. Active as Carbon-monoxide dehydrogenase (acceptor), with EC number 1.2.99.2 Full crystallographic information is available from OCA.
Reference
A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase., Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL, Science. 2002 Oct 18;298(5593):567-72. PMID:12386327
Page seeded by OCA on Thu Feb 21 13:55:45 2008
Categories: Carbon-monoxide dehydrogenase (acceptor) | Moorella thermoacetica | Protein complex | Doukov, T I. | Drennan, C L. | Iverson, T M. | Ragsdale, S W. | Seravalli, J. | ACT | CU1 | NI | SF4 | XCC | Acetyl-coa synthase (acs) | Carbon monoxide dehydrogenase(codh) | Clostridium thermoaceticum | Electron transfer | Helical domain | Hydrophobic co channel | Nickel-coopper-iron-sulfur (ni-cu-fe-s) cluster | Nickel-iron-sulfur cluster (ni-fe-s) | Rossmann fold | Substrate tunnel | Wood-ljundahl pathway
