1mjn
From Proteopedia
(New page: 200px<br /> <applet load="1mjn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mjn, resolution 1.30Å" /> '''Crystal Structure o...) |
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| - | [[Image:1mjn.gif|left|200px]]<br /> | + | [[Image:1mjn.gif|left|200px]]<br /><applet load="1mjn" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1mjn, resolution 1.30Å" /> | caption="1mjn, resolution 1.30Å" /> | ||
'''Crystal Structure of the intermediate affinity aL I domain mutant'''<br /> | '''Crystal Structure of the intermediate affinity aL I domain mutant'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the I domain of integrin alpha L beta 2 bound to the Ig | + | The structure of the I domain of integrin alpha L beta 2 bound to the Ig superfamily ligand ICAM-1 reveals the open ligand binding conformation and the first example of an integrin-IgSF interface. The I domain Mg2+ directly coordinates Glu-34 of ICAM-1, and a dramatic swing of I domain residue Glu-241 enables a critical salt bridge. Liganded and unliganded structures for both high- and intermediate-affinity mutant I domains reveal that ligand binding can induce conformational change in the alpha L I domain and that allosteric signals can convert the closed conformation to intermediate or open conformations without ligand binding. Pulling down on the C-terminal alpha 7 helix with introduced disulfide bonds ratchets the beta 6-alpha 7 loop into three different positions in the closed, intermediate, and open conformations, with a progressive increase in affinity. |
==About this Structure== | ==About this Structure== | ||
| - | 1MJN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1MJN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dong, Y | + | [[Category: Dong, Y C.]] |
| - | [[Category: Jun, C | + | [[Category: Jun, C D.]] |
| - | [[Category: Liu, J | + | [[Category: Liu, J H.]] |
[[Category: McCormack, A.]] | [[Category: McCormack, A.]] | ||
[[Category: Shimaoka, M.]] | [[Category: Shimaoka, M.]] | ||
| - | [[Category: Springer, T | + | [[Category: Springer, T A.]] |
| - | [[Category: Wang, J | + | [[Category: Wang, J H.]] |
[[Category: Xiao, T.]] | [[Category: Xiao, T.]] | ||
| - | [[Category: Yang, Y | + | [[Category: Yang, Y T.]] |
| - | [[Category: Zhang, R | + | [[Category: Zhang, R G.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:51 2008'' |
Revision as of 11:55, 21 February 2008
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Crystal Structure of the intermediate affinity aL I domain mutant
Overview
The structure of the I domain of integrin alpha L beta 2 bound to the Ig superfamily ligand ICAM-1 reveals the open ligand binding conformation and the first example of an integrin-IgSF interface. The I domain Mg2+ directly coordinates Glu-34 of ICAM-1, and a dramatic swing of I domain residue Glu-241 enables a critical salt bridge. Liganded and unliganded structures for both high- and intermediate-affinity mutant I domains reveal that ligand binding can induce conformational change in the alpha L I domain and that allosteric signals can convert the closed conformation to intermediate or open conformations without ligand binding. Pulling down on the C-terminal alpha 7 helix with introduced disulfide bonds ratchets the beta 6-alpha 7 loop into three different positions in the closed, intermediate, and open conformations, with a progressive increase in affinity.
About this Structure
1MJN is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation., Shimaoka M, Xiao T, Liu JH, Yang Y, Dong Y, Jun CD, McCormack A, Zhang R, Joachimiak A, Takagi J, Wang JH, Springer TA, Cell. 2003 Jan 10;112(1):99-111. PMID:12526797
Page seeded by OCA on Thu Feb 21 13:55:51 2008
Categories: Escherichia coli | Single protein | Dong, Y C. | Jun, C D. | Liu, J H. | McCormack, A. | Shimaoka, M. | Springer, T A. | Wang, J H. | Xiao, T. | Yang, Y T. | Zhang, R G. | MG | Rossmann fold
