1mml
From Proteopedia
(New page: 200px<br /><applet load="1mml" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mml, resolution 1.80Å" /> '''MECHANISTIC IMPLICAT...) |
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| - | [[Image:1mml.gif|left|200px]]<br /><applet load="1mml" size=" | + | [[Image:1mml.gif|left|200px]]<br /><applet load="1mml" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mml, resolution 1.80Å" /> | caption="1mml, resolution 1.80Å" /> | ||
'''MECHANISTIC IMPLICATIONS FROM THE STRUCTURE OF A CATALYTIC FRAGMENT OF MMLV REVERSE TRANSCRIPTASE'''<br /> | '''MECHANISTIC IMPLICATIONS FROM THE STRUCTURE OF A CATALYTIC FRAGMENT OF MMLV REVERSE TRANSCRIPTASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | BACKGROUND: Reverse transcriptase (RT) converts the single-stranded RNA | + | BACKGROUND: Reverse transcriptase (RT) converts the single-stranded RNA genome of a retrovirus into a double-stranded DNA copy for integration into the host genome. This process requires ribonuclease H as well as RNA- and DNA-directed DNA polymerase activities. Although the overall organization of HIV-1 RT is known from previously reported crystal structures, no structure of a complex including a metal ion, which is essential for its catalytic activity, has been reported. RESULTS: Here we describe the structures at 1.8 Angstrum resolution of a catalytically active fragment of RT from Moloney murine leukemia virus (MMLV) and at 2.6 Angstrum of a complex of this fragment with Mn2+ coordinated in the polymerase active site. On the basis of similarities with HIV-1 RT and rat DNA polymerase beta, we have modeled template/primer and deoxyribonucleoside 5'-triphosphate substrates into the MMLV RT structure. CONCLUSIONS: Our model, in the context of the disposition of evolutionarily conserved residues seen here at high resolution, provides new insights into the mechanisms of catalysis, fidelity, processivity and discrimination between deoxyribose and ribose nucleotides. |
==About this Structure== | ==About this Structure== | ||
| - | 1MML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http:// | + | 1MML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MML OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Moloney murine leukemia virus]] | [[Category: Moloney murine leukemia virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Georgiadis, M | + | [[Category: Georgiadis, M M.]] |
| - | [[Category: Goff, S | + | [[Category: Goff, S P.]] |
| - | [[Category: Hendrickson, W | + | [[Category: Hendrickson, W A.]] |
| - | [[Category: Jessen, S | + | [[Category: Jessen, S M.]] |
| - | [[Category: Ogata, C | + | [[Category: Ogata, C M.]] |
[[Category: Telesnitsky, A.]] | [[Category: Telesnitsky, A.]] | ||
[[Category: reverse transcriptase]] | [[Category: reverse transcriptase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:56:42 2008'' |
Revision as of 11:56, 21 February 2008
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MECHANISTIC IMPLICATIONS FROM THE STRUCTURE OF A CATALYTIC FRAGMENT OF MMLV REVERSE TRANSCRIPTASE
Overview
BACKGROUND: Reverse transcriptase (RT) converts the single-stranded RNA genome of a retrovirus into a double-stranded DNA copy for integration into the host genome. This process requires ribonuclease H as well as RNA- and DNA-directed DNA polymerase activities. Although the overall organization of HIV-1 RT is known from previously reported crystal structures, no structure of a complex including a metal ion, which is essential for its catalytic activity, has been reported. RESULTS: Here we describe the structures at 1.8 Angstrum resolution of a catalytically active fragment of RT from Moloney murine leukemia virus (MMLV) and at 2.6 Angstrum of a complex of this fragment with Mn2+ coordinated in the polymerase active site. On the basis of similarities with HIV-1 RT and rat DNA polymerase beta, we have modeled template/primer and deoxyribonucleoside 5'-triphosphate substrates into the MMLV RT structure. CONCLUSIONS: Our model, in the context of the disposition of evolutionarily conserved residues seen here at high resolution, provides new insights into the mechanisms of catalysis, fidelity, processivity and discrimination between deoxyribose and ribose nucleotides.
About this Structure
1MML is a Single protein structure of sequence from Moloney murine leukemia virus. Full crystallographic information is available from OCA.
Reference
Mechanistic implications from the structure of a catalytic fragment of Moloney murine leukemia virus reverse transcriptase., Georgiadis MM, Jessen SM, Ogata CM, Telesnitsky A, Goff SP, Hendrickson WA, Structure. 1995 Sep 15;3(9):879-92. PMID:8535782
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