1mnp
From Proteopedia
(New page: 200px<br /><applet load="1mnp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mnp, resolution 2.0Å" /> '''MANGANESE PEROXIDASE'...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1mnp.gif|left|200px]]<br /><applet load="1mnp" size=" | + | [[Image:1mnp.gif|left|200px]]<br /><applet load="1mnp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mnp, resolution 2.0Å" /> | caption="1mnp, resolution 2.0Å" /> | ||
'''MANGANESE PEROXIDASE'''<br /> | '''MANGANESE PEROXIDASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Manganese peroxidase from the white rot basidiomycete Phanerochaete | + | Manganese peroxidase from the white rot basidiomycete Phanerochaete chrysosporium has been crystallized in a form suitable for high-resolution X-ray structure determination. Crystals were grown from solutions containing 30% polyethylene glycol 8000, ammonium sulfate and cacodylate buffer at pH 6.5, using macroseeding techniques. A complete data set has been obtained to 2.06 A resolution. The data can be indexed in space group P1 with a = 45.96 A, b = 53.77 A, c = 84.87 A, alpha = 97.01 degrees, beta = 105.72 degrees and gamma = 90.1 degrees, with two peroxidase molecules per asymmetric unit, or in space group C2 with a = 163.23 A, b = 45.97 A, c = 53.72 A and beta = 97.16 degrees, with only one molecule in the assymetric unit. Lignin peroxidase, which shares about 57% sequence identity with manganese peroxidase, was used as a probe for molecular replacement. Unique rotation and translation solutions have been obtained in space groups P1 and C2. The structure has been partially refined in space group C2 to R = 0.22 for data between 10 and 2.06 A. |
==About this Structure== | ==About this Structure== | ||
| - | 1MNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with CA, MN and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] Full crystallographic information is available from [http:// | + | 1MNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNP OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Phanerochaete chrysosporium]] | [[Category: Phanerochaete chrysosporium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Poulos, T | + | [[Category: Poulos, T L.]] |
[[Category: Sundaramoorthy, M.]] | [[Category: Sundaramoorthy, M.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| Line 21: | Line 21: | ||
[[Category: heme peroxidase]] | [[Category: heme peroxidase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:03 2008'' |
Revision as of 11:57, 21 February 2008
|
MANGANESE PEROXIDASE
Overview
Manganese peroxidase from the white rot basidiomycete Phanerochaete chrysosporium has been crystallized in a form suitable for high-resolution X-ray structure determination. Crystals were grown from solutions containing 30% polyethylene glycol 8000, ammonium sulfate and cacodylate buffer at pH 6.5, using macroseeding techniques. A complete data set has been obtained to 2.06 A resolution. The data can be indexed in space group P1 with a = 45.96 A, b = 53.77 A, c = 84.87 A, alpha = 97.01 degrees, beta = 105.72 degrees and gamma = 90.1 degrees, with two peroxidase molecules per asymmetric unit, or in space group C2 with a = 163.23 A, b = 45.97 A, c = 53.72 A and beta = 97.16 degrees, with only one molecule in the assymetric unit. Lignin peroxidase, which shares about 57% sequence identity with manganese peroxidase, was used as a probe for molecular replacement. Unique rotation and translation solutions have been obtained in space groups P1 and C2. The structure has been partially refined in space group C2 to R = 0.22 for data between 10 and 2.06 A.
About this Structure
1MNP is a Single protein structure of sequence from Phanerochaete chrysosporium with , and as ligands. Active as Peroxidase, with EC number 1.11.1.7 Full crystallographic information is available from OCA.
Reference
Preliminary crystallographic analysis of manganese peroxidase from Phanerochaete chrysosporium., Sundaramoorthy M, Kishi K, Gold MH, Poulos TL, J Mol Biol. 1994 May 20;238(5):845-8. PMID:8182752
Page seeded by OCA on Thu Feb 21 13:57:03 2008
