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1mom
From Proteopedia
(New page: 200px<br /><applet load="1mom" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mom, resolution 2.16Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1mom.jpg|left|200px]]<br /><applet load="1mom" size=" | + | [[Image:1mom.jpg|left|200px]]<br /><applet load="1mom" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mom, resolution 2.16Å" /> | caption="1mom, resolution 2.16Å" /> | ||
'''CRYSTAL STRUCTURE OF MOMORDIN, A TYPE I RIBOSOME INACTIVATING PROTEIN FROM THE SEEDS OF MOMORDICA CHARANTIA'''<br /> | '''CRYSTAL STRUCTURE OF MOMORDIN, A TYPE I RIBOSOME INACTIVATING PROTEIN FROM THE SEEDS OF MOMORDICA CHARANTIA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A type I ribosome-inactivating protein, extracted and purified from M. | + | A type I ribosome-inactivating protein, extracted and purified from M. charantia seeds, was crystallised by vapour diffusion with polyethylene glycol at pH 7.2. X-ray data were collected to 2.1 A resolution and the structure solved by molecular replacement using the A-chain coordinates of ricin. The overall fold of the protein is similar to ricin but there are differences in secondary structure, on the surface and in the active site cleft. These differences are probably due in part to the evolution of the protein without a B-chain partner. The most extensive reorganisation occurs at the C-terminus whereas Tyr70 shows the greatest change in the active site cleft. |
==About this Structure== | ==About this Structure== | ||
| - | 1MOM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http:// | + | 1MOM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rRNA N-glycosylase]] | [[Category: rRNA N-glycosylase]] | ||
[[Category: Husain, J.]] | [[Category: Husain, J.]] | ||
| - | [[Category: Tickle, I | + | [[Category: Tickle, I J.]] |
| - | [[Category: Wood, S | + | [[Category: Wood, S P.]] |
[[Category: protein synthesis inhibitor(toxin)]] | [[Category: protein synthesis inhibitor(toxin)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:22 2008'' |
Revision as of 11:57, 21 February 2008
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CRYSTAL STRUCTURE OF MOMORDIN, A TYPE I RIBOSOME INACTIVATING PROTEIN FROM THE SEEDS OF MOMORDICA CHARANTIA
Overview
A type I ribosome-inactivating protein, extracted and purified from M. charantia seeds, was crystallised by vapour diffusion with polyethylene glycol at pH 7.2. X-ray data were collected to 2.1 A resolution and the structure solved by molecular replacement using the A-chain coordinates of ricin. The overall fold of the protein is similar to ricin but there are differences in secondary structure, on the surface and in the active site cleft. These differences are probably due in part to the evolution of the protein without a B-chain partner. The most extensive reorganisation occurs at the C-terminus whereas Tyr70 shows the greatest change in the active site cleft.
About this Structure
1MOM is a Single protein structure of sequence from [1]. Active as rRNA N-glycosylase, with EC number 3.2.2.22 Full crystallographic information is available from OCA.
Reference
Crystal structure of momordin, a type I ribosome inactivating protein from the seeds of Momordica charantia., Husain J, Tickle IJ, Wood SP, FEBS Lett. 1994 Apr 4;342(2):154-8. PMID:8143869
Page seeded by OCA on Thu Feb 21 13:57:22 2008
