1mt4
From Proteopedia
(New page: 200px<br /><applet load="1mt4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mt4" /> '''Structure of 23S ribosomal RNA hairpin 35'''...) |
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| - | [[Image:1mt4.gif|left|200px]]<br /><applet load="1mt4" size=" | + | [[Image:1mt4.gif|left|200px]]<br /><applet load="1mt4" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Structure of 23S ribosomal RNA hairpin 35'''<br /> | '''Structure of 23S ribosomal RNA hairpin 35'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The bacterial rRNA methyltransferase RlmAII (formerly TlrB) contributes to | + | The bacterial rRNA methyltransferase RlmAII (formerly TlrB) contributes to resistance against tylosin-like 16-membered ring macrolide antibiotics. RlmAII was originally discovered in the tylosin-producer Streptomyces fradiae, and members of this subclass of methyltransferases have subsequently been found in other Gram-positive bacteria, including Streptococcus pneumoniae. In all cases, RlmAII methylates 23S rRNA at nucleotide G748, which is situated in a stem-loop (hairpin 35) at the macrolide binding site of the ribosome. The conformation of hairpin 35 recognized by RlmAII is shown here by NMR spectroscopy to resemble the anticodon loop of tRNA. The loop folds independently of the rest of the 23S rRNA, and is stabilized by a non-canonical G-A pair and a U-turn motif, rendering G748 accessible. Binding of S.pneumoniae RlmAII induces changes in NMR signals at specific nucleotides that are involved in the methyltransferase-RNA interaction. The conformation of hairpin 35 that interacts with RlmAII is radically different from the structure this hairpin adopts within the 50S subunit. This indicates that the hairpin undergoes major structural rearrangement upon interaction with ribosomal proteins during 50S assembly. |
==About this Structure== | ==About this Structure== | ||
| - | 1MT4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1MT4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MT4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Guittet, E.]] | [[Category: Guittet, E.]] | ||
[[Category: Lebars, I.]] | [[Category: Lebars, I.]] | ||
| - | [[Category: Stenholm, A | + | [[Category: Stenholm, A R.]] |
[[Category: Yoshizawa, S.]] | [[Category: Yoshizawa, S.]] | ||
[[Category: ribosomal rna]] | [[Category: ribosomal rna]] | ||
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[[Category: u-turn]] | [[Category: u-turn]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:44 2008'' |
Revision as of 11:58, 21 February 2008
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Structure of 23S ribosomal RNA hairpin 35
Overview
The bacterial rRNA methyltransferase RlmAII (formerly TlrB) contributes to resistance against tylosin-like 16-membered ring macrolide antibiotics. RlmAII was originally discovered in the tylosin-producer Streptomyces fradiae, and members of this subclass of methyltransferases have subsequently been found in other Gram-positive bacteria, including Streptococcus pneumoniae. In all cases, RlmAII methylates 23S rRNA at nucleotide G748, which is situated in a stem-loop (hairpin 35) at the macrolide binding site of the ribosome. The conformation of hairpin 35 recognized by RlmAII is shown here by NMR spectroscopy to resemble the anticodon loop of tRNA. The loop folds independently of the rest of the 23S rRNA, and is stabilized by a non-canonical G-A pair and a U-turn motif, rendering G748 accessible. Binding of S.pneumoniae RlmAII induces changes in NMR signals at specific nucleotides that are involved in the methyltransferase-RNA interaction. The conformation of hairpin 35 that interacts with RlmAII is radically different from the structure this hairpin adopts within the 50S subunit. This indicates that the hairpin undergoes major structural rearrangement upon interaction with ribosomal proteins during 50S assembly.
About this Structure
1MT4 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII., Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S, Fourmy D, EMBO J. 2003 Jan 15;22(2):183-92. PMID:12514124
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