1mtl
From Proteopedia
(New page: 200px<br /><applet load="1mtl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mtl, resolution 2.80Å" /> '''Non-productive MUG-D...) |
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| - | [[Image:1mtl.jpg|left|200px]]<br /><applet load="1mtl" size=" | + | [[Image:1mtl.jpg|left|200px]]<br /><applet load="1mtl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mtl, resolution 2.80Å" /> | caption="1mtl, resolution 2.80Å" /> | ||
'''Non-productive MUG-DNA complex'''<br /> | '''Non-productive MUG-DNA complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Base-excision of a self-complementary oligonucleotide with central G:T | + | Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis. |
==About this Structure== | ==About this Structure== | ||
| - | 1MTL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1MTL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barlow, T.]] | [[Category: Barlow, T.]] | ||
| - | [[Category: Barrett, T | + | [[Category: Barrett, T E.]] |
[[Category: Brown, T.]] | [[Category: Brown, T.]] | ||
[[Category: Jiricny, J.]] | [[Category: Jiricny, J.]] | ||
| - | [[Category: Pearl, L | + | [[Category: Pearl, L H.]] |
[[Category: Savva, R.]] | [[Category: Savva, R.]] | ||
[[Category: cis-platin]] | [[Category: cis-platin]] | ||
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[[Category: non-productive]] | [[Category: non-productive]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:58:51 2008'' |
Revision as of 11:58, 21 February 2008
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Non-productive MUG-DNA complex
Overview
Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis.
About this Structure
1MTL is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct., Barrett TE, Savva R, Barlow T, Brown T, Jiricny J, Pearl LH, Nat Struct Biol. 1998 Aug;5(8):697-701. PMID:9699633
Page seeded by OCA on Thu Feb 21 13:58:51 2008
