1mxi
From Proteopedia
(New page: 200px<br /><applet load="1mxi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mxi, resolution 1.7Å" /> '''Structure of YibK fro...) |
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| - | [[Image:1mxi.gif|left|200px]]<br /><applet load="1mxi" size=" | + | [[Image:1mxi.gif|left|200px]]<br /><applet load="1mxi" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mxi, resolution 1.7Å" /> | caption="1mxi, resolution 1.7Å" /> | ||
'''Structure of YibK from Haemophilus influenzae (HI0766): a Methyltransferase with a Cofactor Bound at a Site Formed by a Knot'''<br /> | '''Structure of YibK from Haemophilus influenzae (HI0766): a Methyltransferase with a Cofactor Bound at a Site Formed by a Knot'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structures of YibK from Haemophilus influenzae (HI0766) have | + | The crystal structures of YibK from Haemophilus influenzae (HI0766) have been determined with and without bound cofactor product S-adenosylhomocysteine (AdoHcy) at 1.7 and 2.0 A resolution, respectively. The molecule adopts an alpha/beta fold, with a topology that differs from that of the classical methyltransferases. Most notably, HI0766 contains a striking knot that forms the binding crevice for the cofactor. The knot formation is correlated with an alternative arrangement of the secondary structure units compared with the classical methyltransferases. Two loop regions undergo conformational changes upon AdoHcy binding. In contrast to the extended conformation of the cofactor seen in the classical methyltransferase structures, AdoHcy binds to HI0766 in a bent conformation. HI0766 and its close sequence relatives are all shorter versions of the more remotely related rRNA/tRNA methyltransferases of the spoU sequence family. We propose that the spoU sequence family contains the same core domain for cofactor binding as HI0766 but has an additional domain for substrate binding. The substrate-binding domain is absent in HI0766 sequence family and may be provided by another Haemophilus influenzae partner protein, which is yet to be identified. |
==About this Structure== | ==About this Structure== | ||
| - | 1MXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with IOD and SAH as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1MXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with <scene name='pdbligand=IOD:'>IOD</scene> and <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MXI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Howard, A.]] | [[Category: Howard, A.]] | ||
[[Category: Lim, K.]] | [[Category: Lim, K.]] | ||
| - | [[Category: S2F, Structure | + | [[Category: S2F, Structure 2.Function Project.]] |
[[Category: Tempczyk, A.]] | [[Category: Tempczyk, A.]] | ||
[[Category: Toedt, J.]] | [[Category: Toedt, J.]] | ||
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[[Category: structure 2 function project]] | [[Category: structure 2 function project]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:07 2008'' |
Revision as of 12:00, 21 February 2008
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Structure of YibK from Haemophilus influenzae (HI0766): a Methyltransferase with a Cofactor Bound at a Site Formed by a Knot
Overview
The crystal structures of YibK from Haemophilus influenzae (HI0766) have been determined with and without bound cofactor product S-adenosylhomocysteine (AdoHcy) at 1.7 and 2.0 A resolution, respectively. The molecule adopts an alpha/beta fold, with a topology that differs from that of the classical methyltransferases. Most notably, HI0766 contains a striking knot that forms the binding crevice for the cofactor. The knot formation is correlated with an alternative arrangement of the secondary structure units compared with the classical methyltransferases. Two loop regions undergo conformational changes upon AdoHcy binding. In contrast to the extended conformation of the cofactor seen in the classical methyltransferase structures, AdoHcy binds to HI0766 in a bent conformation. HI0766 and its close sequence relatives are all shorter versions of the more remotely related rRNA/tRNA methyltransferases of the spoU sequence family. We propose that the spoU sequence family contains the same core domain for cofactor binding as HI0766 but has an additional domain for substrate binding. The substrate-binding domain is absent in HI0766 sequence family and may be provided by another Haemophilus influenzae partner protein, which is yet to be identified.
About this Structure
1MXI is a Single protein structure of sequence from Haemophilus influenzae with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the YibK methyltransferase from Haemophilus influenzae (HI0766): a cofactor bound at a site formed by a knot., Lim K, Zhang H, Tempczyk A, Krajewski W, Bonander N, Toedt J, Howard A, Eisenstein E, Herzberg O, Proteins. 2003 Apr 1;51(1):56-67. PMID:12596263
Page seeded by OCA on Thu Feb 21 14:00:07 2008
Categories: Haemophilus influenzae | Single protein | Bonander, N. | Eisenstein, E. | Herzberg, O. | Howard, A. | Lim, K. | S2F, Structure 2.Function Project. | Tempczyk, A. | Toedt, J. | Zhang, H. | IOD | SAH | Methyltransferase | S-adenosylhomocysteine | S2f | Spou family | Structural genomics | Structure 2 function project
