1mzj
From Proteopedia
(New page: 200px<br /><applet load="1mzj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mzj, resolution 2.1Å" /> '''Crystal Structure of ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1mzj.jpg|left|200px]]<br /><applet load="1mzj" size=" | + | [[Image:1mzj.jpg|left|200px]]<br /><applet load="1mzj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mzj, resolution 2.1Å" /> | caption="1mzj, resolution 2.1Å" /> | ||
'''Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway'''<br /> | '''Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway'''<br /> | ||
==Overview== | ==Overview== | ||
| - | ZhuH is a priming ketosynthase that initiates the elongation of the | + | ZhuH is a priming ketosynthase that initiates the elongation of the polyketide chain in the biosynthetic pathway of a type II polyketide, R1128. The crystal structure of ZhuH in complex with the priming substrate acetyl-CoA reveals an extensive loop region at the dimer interface that appears to affect the selectivity for the primer unit. Acetyl-CoA is bound in a 20 A-long channel, which placed the acetyl group against the catalytic triad. Analysis of the primer unit binding site in ZhuH suggests that it can accommodate acyl chains that are two to four carbons long. Selectivity and primer unit size appear to involve the side chains of three residues on the loops close to the dimer interface that constitute the bottom of the substrate binding pocket. |
==About this Structure== | ==About this Structure== | ||
| - | 1MZJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r22 Streptomyces sp. r22] with COA and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] Full crystallographic information is available from [http:// | + | 1MZJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r22 Streptomyces sp. r22] with <scene name='pdbligand=COA:'>COA</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZJ OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces sp. r22]] | [[Category: Streptomyces sp. r22]] | ||
| - | [[Category: Connell, J | + | [[Category: Connell, J O.]] |
[[Category: Keatinge-Clay, A.]] | [[Category: Keatinge-Clay, A.]] | ||
[[Category: Khosla, C.]] | [[Category: Khosla, C.]] | ||
| - | [[Category: Meadows, E | + | [[Category: Meadows, E S.]] |
| - | [[Category: Miercke, L | + | [[Category: Miercke, L J.W.]] |
[[Category: Pan, H.]] | [[Category: Pan, H.]] | ||
| - | [[Category: Stroud, R | + | [[Category: Stroud, R M.]] |
| - | [[Category: Tsai, S | + | [[Category: Tsai, S C.]] |
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: COA]] | [[Category: COA]] | ||
| Line 29: | Line 29: | ||
[[Category: catalytic triad]] | [[Category: catalytic triad]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:43 2008'' |
Revision as of 12:00, 21 February 2008
|
Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway
Overview
ZhuH is a priming ketosynthase that initiates the elongation of the polyketide chain in the biosynthetic pathway of a type II polyketide, R1128. The crystal structure of ZhuH in complex with the priming substrate acetyl-CoA reveals an extensive loop region at the dimer interface that appears to affect the selectivity for the primer unit. Acetyl-CoA is bound in a 20 A-long channel, which placed the acetyl group against the catalytic triad. Analysis of the primer unit binding site in ZhuH suggests that it can accommodate acyl chains that are two to four carbons long. Selectivity and primer unit size appear to involve the side chains of three residues on the loops close to the dimer interface that constitute the bottom of the substrate binding pocket.
About this Structure
1MZJ is a Single protein structure of sequence from Streptomyces sp. r22 with and as ligands. Active as Beta-ketoacyl-acyl-carrier-protein synthase I, with EC number 2.3.1.41 Full crystallographic information is available from OCA.
Reference
Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway., Pan H, Tsai S, Meadows ES, Miercke LJ, Keatinge-Clay AT, O'Connell J, Khosla C, Stroud RM, Structure. 2002 Nov;10(11):1559-68. PMID:12429097
Page seeded by OCA on Thu Feb 21 14:00:43 2008
Categories: Beta-ketoacyl-acyl-carrier-protein synthase I | Single protein | Streptomyces sp. r22 | Connell, J O. | Keatinge-Clay, A. | Khosla, C. | Meadows, E S. | Miercke, L J.W. | Pan, H. | Stroud, R M. | Tsai, S C. | ACE | COA | Aromatic polyketide | Beta-ketosynthase | Biosynthetic engineering | Catalytic triad
