1n27
From Proteopedia
(New page: 200px<br /><applet load="1n27" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n27" /> '''Solution structure of the PWWP domain of mou...) |
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| - | [[Image:1n27.gif|left|200px]]<br /><applet load="1n27" size=" | + | [[Image:1n27.gif|left|200px]]<br /><applet load="1n27" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of the PWWP domain of mouse Hepatoma-derived growth factor, related protein 3'''<br /> | '''Solution structure of the PWWP domain of mouse Hepatoma-derived growth factor, related protein 3'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Among the many PWWP-containing proteins, the largest group of homologous | + | Among the many PWWP-containing proteins, the largest group of homologous proteins is related to hepatoma-derived growth factor (HDGF). Within a well-conserved region at the extreme N-terminus, HDGF and five HDGF-related proteins (HRPs) always have a PWWP domain, which is a module found in many chromatin-associated proteins. In this study, we determined the solution structure of the PWWP domain of HDGF-related protein-3 (HRP-3) by NMR spectroscopy. The structure consists of a five-stranded beta-barrel with a PWWP-specific long loop connecting beta2 and beta3 (PR-loop), followed by a helical region including two alpha-helices. Its structure was found to have a characteristic solvent-exposed hydrophobic cavity, which is composed of an abundance of aromatic residues in the beta1/beta2 loop (beta-beta arch) and the beta3/beta4 loop. A similar ligand binding cavity occurs at the corresponding position in the Tudor, chromo, and MBT domains, which have structural and probable evolutionary relationships with PWWP domains. These findings suggest that the PWWP domains of the HDGF family bind to some component of chromatin via the cavity. |
==About this Structure== | ==About this Structure== | ||
| - | 1N27 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1N27 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N27 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Koshiba, S.]] | [[Category: Koshiba, S.]] | ||
[[Category: Nameki, N.]] | [[Category: Nameki, N.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Tochio, N.]] | [[Category: Tochio, N.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:25 2008'' |
Revision as of 12:01, 21 February 2008
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Solution structure of the PWWP domain of mouse Hepatoma-derived growth factor, related protein 3
Overview
Among the many PWWP-containing proteins, the largest group of homologous proteins is related to hepatoma-derived growth factor (HDGF). Within a well-conserved region at the extreme N-terminus, HDGF and five HDGF-related proteins (HRPs) always have a PWWP domain, which is a module found in many chromatin-associated proteins. In this study, we determined the solution structure of the PWWP domain of HDGF-related protein-3 (HRP-3) by NMR spectroscopy. The structure consists of a five-stranded beta-barrel with a PWWP-specific long loop connecting beta2 and beta3 (PR-loop), followed by a helical region including two alpha-helices. Its structure was found to have a characteristic solvent-exposed hydrophobic cavity, which is composed of an abundance of aromatic residues in the beta1/beta2 loop (beta-beta arch) and the beta3/beta4 loop. A similar ligand binding cavity occurs at the corresponding position in the Tudor, chromo, and MBT domains, which have structural and probable evolutionary relationships with PWWP domains. These findings suggest that the PWWP domains of the HDGF family bind to some component of chromatin via the cavity.
About this Structure
1N27 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Solution structure of the PWWP domain of the hepatoma-derived growth factor family., Nameki N, Tochio N, Koshiba S, Inoue M, Yabuki T, Aoki M, Seki E, Matsuda T, Fujikura Y, Saito M, Ikari M, Watanabe M, Terada T, Shirouzu M, Yoshida M, Hirota H, Tanaka A, Hayashizaki Y, Guntert P, Kigawa T, Yokoyama S, Protein Sci. 2005 Mar;14(3):756-64. Epub 2005 Feb 2. PMID:15689505
Page seeded by OCA on Thu Feb 21 14:01:25 2008
Categories: Mus musculus | Single protein | Inoue, M. | Kigawa, T. | Kobayashi, N. | Koshiba, S. | Nameki, N. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Tochio, N. | Yokoyama, S. | Hath | Hdgf family | Nuclear translocation | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics
