1n2d
From Proteopedia
(New page: 200px<br /><applet load="1n2d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n2d, resolution 2.00Å" /> '''Ternary complex of M...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1n2d.gif|left|200px]]<br /><applet load="1n2d" size=" | + | [[Image:1n2d.gif|left|200px]]<br /><applet load="1n2d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n2d, resolution 2.00Å" /> | caption="1n2d, resolution 2.00Å" /> | ||
'''Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin'''<br /> | '''Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces | + | Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model. |
==About this Structure== | ==About this Structure== | ||
| - | 1N2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1N2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2D OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Dominguez, R.]] | [[Category: Dominguez, R.]] | ||
| - | [[Category: Lu, R | + | [[Category: Lu, R C.]] |
| - | [[Category: Stafford, W | + | [[Category: Stafford, W F.]] |
[[Category: Terrak, M.]] | [[Category: Terrak, M.]] | ||
[[Category: Wu, G.]] | [[Category: Wu, G.]] | ||
| Line 22: | Line 22: | ||
[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:30 2008'' |
Revision as of 12:01, 21 February 2008
|
Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin
Overview
Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model.
About this Structure
1N2D is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V., Terrak M, Otterbein LR, Wu G, Palecanda LA, Lu RC, Dominguez R, Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 2):1882-5. Epub, 2002 Sep 28. PMID:12351846
Page seeded by OCA on Thu Feb 21 14:01:30 2008
