1n3j
From Proteopedia
(New page: 200px<br /><applet load="1n3j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n3j" /> '''Structure and Substrate of a Histone H3 Lysi...) |
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| - | [[Image:1n3j.jpg|left|200px]]<br /><applet load="1n3j" size=" | + | [[Image:1n3j.jpg|left|200px]]<br /><applet load="1n3j" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1'''<br /> | '''Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Site-specific lysine methylation of histones by SET domains is a hallmark | + | Site-specific lysine methylation of histones by SET domains is a hallmark for epigenetic control of gene transcription in eukaryotic organisms. Here we report that a SET domain protein from Paramecium bursaria chlorella virus can specifically di-methylate Lys27 in histone H3, a modification implicated in gene silencing. The solution structure of the viral SET domain reveals a butterfly-shaped head-to-head symmetric dimer different from other known protein methyltransferases. Each subunit consists of a Greek-key antiparallel beta-barrel and a three-stranded open-faced sandwich that mediates the dimer interface. Cofactor S-adenosyl-L-methionine (SAM) binds at the opening of the beta-barrel, and amino acids C-terminal to Lys27 in H3 and in the flexible C-terminal tail of the enzyme confer the specificity of this viral histone methyltransferase. |
==About this Structure== | ==About this Structure== | ||
| - | 1N3J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http:// | + | 1N3J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Farooq, A.]] | [[Category: Farooq, A.]] | ||
| - | [[Category: Koch, A | + | [[Category: Koch, A W.]] |
| - | [[Category: Manzur, K | + | [[Category: Manzur, K L.]] |
[[Category: Plotnikova, O.]] | [[Category: Plotnikova, O.]] | ||
[[Category: Sachchidanand]] | [[Category: Sachchidanand]] | ||
[[Category: Zeng, L.]] | [[Category: Zeng, L.]] | ||
| - | [[Category: Zhou, M | + | [[Category: Zhou, M M.]] |
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
[[Category: homodimer]] | [[Category: homodimer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:49 2008'' |
Revision as of 12:01, 21 February 2008
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Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1
Overview
Site-specific lysine methylation of histones by SET domains is a hallmark for epigenetic control of gene transcription in eukaryotic organisms. Here we report that a SET domain protein from Paramecium bursaria chlorella virus can specifically di-methylate Lys27 in histone H3, a modification implicated in gene silencing. The solution structure of the viral SET domain reveals a butterfly-shaped head-to-head symmetric dimer different from other known protein methyltransferases. Each subunit consists of a Greek-key antiparallel beta-barrel and a three-stranded open-faced sandwich that mediates the dimer interface. Cofactor S-adenosyl-L-methionine (SAM) binds at the opening of the beta-barrel, and amino acids C-terminal to Lys27 in H3 and in the flexible C-terminal tail of the enzyme confer the specificity of this viral histone methyltransferase.
About this Structure
1N3J is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3., Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM, Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185
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