1n3k
From Proteopedia
(New page: 200px<br /><applet load="1n3k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n3k" /> '''Solution structure of phosphoprotein enriche...) |
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| - | [[Image:1n3k.jpg|left|200px]]<br /><applet load="1n3k" size=" | + | [[Image:1n3k.jpg|left|200px]]<br /><applet load="1n3k" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of phosphoprotein enriched in astrocytes 15 kDa (PEA-15)'''<br /> | '''Solution structure of phosphoprotein enriched in astrocytes 15 kDa (PEA-15)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | PEA-15 is a multifunctional protein that modulates signaling pathways | + | PEA-15 is a multifunctional protein that modulates signaling pathways which control cell proliferation and cell death. In particular, PEA-15 regulates the actions of the ERK MAP kinase cascade by binding to ERK and altering its subcellular localization. The three-dimensional structure of PEA-15 has been determined using NMR spectroscopy and its interaction with ERK defined by characterization of mutants that modulate ERK function. PEA-15 is composed of an N-terminal death effector domain (DED) and a C-terminal tail of irregular structure. NMR 'footprinting' and mutagenesis identified elements of both the DED and tail that are required for ERK binding. Comparison of the DED-binding surface for ERK2 with the death domain (DD)-binding surface of Drosophila Tube revealed an unexpected similarity between the interaction modes of the DD and DED motifs in these proteins. Despite a lack of functional or sequence similarity between PEA-15 and Tube, these proteins utilize a common surface of the structurally similar DD and DED to recognize functionally diverse targets. |
==About this Structure== | ==About this Structure== | ||
| - | 1N3K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http:// | + | 1N3K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ginsberg, M | + | [[Category: Ginsberg, M H.]] |
| - | [[Category: Hill, J | + | [[Category: Hill, J M.]] |
| - | [[Category: Ramos, J | + | [[Category: Ramos, J W.]] |
[[Category: Vaidyanathan, H.]] | [[Category: Vaidyanathan, H.]] | ||
| - | [[Category: Werner, M | + | [[Category: Werner, M H.]] |
[[Category: death effector domain]] | [[Category: death effector domain]] | ||
[[Category: six helix bundle]] | [[Category: six helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:53 2008'' |
Revision as of 12:01, 21 February 2008
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Solution structure of phosphoprotein enriched in astrocytes 15 kDa (PEA-15)
Overview
PEA-15 is a multifunctional protein that modulates signaling pathways which control cell proliferation and cell death. In particular, PEA-15 regulates the actions of the ERK MAP kinase cascade by binding to ERK and altering its subcellular localization. The three-dimensional structure of PEA-15 has been determined using NMR spectroscopy and its interaction with ERK defined by characterization of mutants that modulate ERK function. PEA-15 is composed of an N-terminal death effector domain (DED) and a C-terminal tail of irregular structure. NMR 'footprinting' and mutagenesis identified elements of both the DED and tail that are required for ERK binding. Comparison of the DED-binding surface for ERK2 with the death domain (DD)-binding surface of Drosophila Tube revealed an unexpected similarity between the interaction modes of the DD and DED motifs in these proteins. Despite a lack of functional or sequence similarity between PEA-15 and Tube, these proteins utilize a common surface of the structurally similar DD and DED to recognize functionally diverse targets.
About this Structure
1N3K is a Single protein structure of sequence from Cricetulus griseus. Full crystallographic information is available from OCA.
Reference
Recognition of ERK MAP kinase by PEA-15 reveals a common docking site within the death domain and death effector domain., Hill JM, Vaidyanathan H, Ramos JW, Ginsberg MH, Werner MH, EMBO J. 2002 Dec 2;21(23):6494-504. PMID:12456656
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