1n47
From Proteopedia
(New page: 200px<br /><applet load="1n47" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n47, resolution 2.70Å" /> '''Isolectin B4 from Vi...) |
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| - | [[Image:1n47.gif|left|200px]]<br /><applet load="1n47" size=" | + | [[Image:1n47.gif|left|200px]]<br /><applet load="1n47" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n47, resolution 2.70Å" /> | caption="1n47, resolution 2.70Å" /> | ||
'''Isolectin B4 from Vicia villosa in complex with the Tn antigen'''<br /> | '''Isolectin B4 from Vicia villosa in complex with the Tn antigen'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the tetrameric Vicia villosa isolectin B4 (VVLB4) in | + | The structure of the tetrameric Vicia villosa isolectin B4 (VVLB4) in complex with a cancer antigen, the Tn glycopeptide (GalNAc-O-Ser), was determined at 2.7 A resolution. The N-acetylgalactoside moiety of the ligand binds to the primary combining site of VVLB4 in a similar way as observed for other Gal/GalNAc-specific plant lectins. The amino acid moiety of the Tn antigen is largely exposed to the solvent and makes few contacts with the protein. The structure of the complex provides a framework to understand the differences in the strength of VVLB4 binding to different sugars and emphasizes the role of a single protein residue, Tyr127, as a structural determinant of Tn-binding specificity. |
==About this Structure== | ==About this Structure== | ||
| - | 1N47 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vicia_villosa Vicia villosa] with CA, MN and TNR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1N47 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vicia_villosa Vicia villosa] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=TNR:'>TNR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N47 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vicia villosa]] | [[Category: Vicia villosa]] | ||
| - | [[Category: Alzari, P | + | [[Category: Alzari, P M.]] |
[[Category: Babino, A.]] | [[Category: Babino, A.]] | ||
[[Category: Bay, S.]] | [[Category: Bay, S.]] | ||
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[[Category: vicia villosa lectin]] | [[Category: vicia villosa lectin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:06 2008'' |
Revision as of 12:02, 21 February 2008
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Isolectin B4 from Vicia villosa in complex with the Tn antigen
Overview
The structure of the tetrameric Vicia villosa isolectin B4 (VVLB4) in complex with a cancer antigen, the Tn glycopeptide (GalNAc-O-Ser), was determined at 2.7 A resolution. The N-acetylgalactoside moiety of the ligand binds to the primary combining site of VVLB4 in a similar way as observed for other Gal/GalNAc-specific plant lectins. The amino acid moiety of the Tn antigen is largely exposed to the solvent and makes few contacts with the protein. The structure of the complex provides a framework to understand the differences in the strength of VVLB4 binding to different sugars and emphasizes the role of a single protein residue, Tyr127, as a structural determinant of Tn-binding specificity.
About this Structure
1N47 is a Protein complex structure of sequences from Vicia villosa with , and as ligands. Full crystallographic information is available from OCA.
Reference
The crystal structure of a plant lectin in complex with the Tn antigen., Babino A, Tello D, Rojas A, Bay S, Osinaga E, Alzari PM, FEBS Lett. 2003 Feb 11;536(1-3):106-10. PMID:12586347
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