1n5m
From Proteopedia
(New page: 200px<br /><applet load="1n5m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n5m, resolution 2.20Å" /> '''Crystal structure of...) |
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| - | [[Image:1n5m.gif|left|200px]]<br /><applet load="1n5m" size=" | + | [[Image:1n5m.gif|left|200px]]<br /><applet load="1n5m" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n5m, resolution 2.20Å" /> | caption="1n5m, resolution 2.20Å" /> | ||
'''Crystal structure of the mouse acetylcholinesterase-gallamine complex'''<br /> | '''Crystal structure of the mouse acetylcholinesterase-gallamine complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The peripheral anionic site on acetylcholinesterase (AChE), located at the | + | The peripheral anionic site on acetylcholinesterase (AChE), located at the active center gorge entry, encompasses overlapping binding sites for allosteric activators and inhibitors; yet, the molecular mechanisms coupling this site to the active center at the gorge base to modulate catalysis remain unclear. The peripheral site has also been proposed to be involved in heterologous protein associations occurring during synaptogenesis or upon neurodegeneration. A novel crystal form of mouse AChE, combined with spectrophotometric analyses of the crystals, enabled us to solve unique structures of AChE with a free peripheral site, and as three complexes with peripheral site inhibitors: the phenylphenanthridinium ligands, decidium and propidium, and the pyrogallol ligand, gallamine, at 2.20-2.35 A resolution. Comparison with structures of AChE complexes with the peptide fasciculin or with organic bifunctional inhibitors unveils new structural determinants contributing to ligand interactions at the peripheral site, and permits a detailed topographic delineation of this site. Hence, these structures provide templates for designing compounds directed to the enzyme surface that modulate specific surface interactions controlling catalytic activity and non-catalytic heterologous protein associations. |
==About this Structure== | ==About this Structure== | ||
| - | 1N5M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG, CO3, IOD, P6G, PG4 and GMN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] Full crystallographic information is available from [http:// | + | 1N5M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CO3:'>CO3</scene>, <scene name='pdbligand=IOD:'>IOD</scene>, <scene name='pdbligand=P6G:'>P6G</scene>, <scene name='pdbligand=PG4:'>PG4</scene> and <scene name='pdbligand=GMN:'>GMN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: serine esterase]] | [[Category: serine esterase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:27 2008'' |
Revision as of 12:02, 21 February 2008
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Crystal structure of the mouse acetylcholinesterase-gallamine complex
Overview
The peripheral anionic site on acetylcholinesterase (AChE), located at the active center gorge entry, encompasses overlapping binding sites for allosteric activators and inhibitors; yet, the molecular mechanisms coupling this site to the active center at the gorge base to modulate catalysis remain unclear. The peripheral site has also been proposed to be involved in heterologous protein associations occurring during synaptogenesis or upon neurodegeneration. A novel crystal form of mouse AChE, combined with spectrophotometric analyses of the crystals, enabled us to solve unique structures of AChE with a free peripheral site, and as three complexes with peripheral site inhibitors: the phenylphenanthridinium ligands, decidium and propidium, and the pyrogallol ligand, gallamine, at 2.20-2.35 A resolution. Comparison with structures of AChE complexes with the peptide fasciculin or with organic bifunctional inhibitors unveils new structural determinants contributing to ligand interactions at the peripheral site, and permits a detailed topographic delineation of this site. Hence, these structures provide templates for designing compounds directed to the enzyme surface that modulate specific surface interactions controlling catalytic activity and non-catalytic heterologous protein associations.
About this Structure
1N5M is a Single protein structure of sequence from Mus musculus with , , , , and as ligands. Active as Acetylcholinesterase, with EC number 3.1.1.7 Full crystallographic information is available from OCA.
Reference
Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site., Bourne Y, Taylor P, Radic Z, Marchot P, EMBO J. 2003 Jan 2;22(1):1-12. PMID:12505979
Page seeded by OCA on Thu Feb 21 14:02:27 2008
Categories: Acetylcholinesterase | Mus musculus | Single protein | Bourne, Y. | Marchot, P. | Radic, Z. | Taylor, P. | CO3 | GMN | IOD | NAG | P6G | PG4 | Glycosylated protein | Homodimer | Hydrolase | Hydrolase fold | Serine esterase
