1n5x
From Proteopedia
(New page: 200px<br /><applet load="1n5x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n5x, resolution 2.80Å" /> '''Xanthine Dehydrogena...) |
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| - | [[Image:1n5x.gif|left|200px]]<br /><applet load="1n5x" size=" | + | [[Image:1n5x.gif|left|200px]]<br /><applet load="1n5x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n5x, resolution 2.80Å" /> | caption="1n5x, resolution 2.80Å" /> | ||
'''Xanthine Dehydrogenase from Bovine Milk with Inhibitor TEI-6720 Bound'''<br /> | '''Xanthine Dehydrogenase from Bovine Milk with Inhibitor TEI-6720 Bound'''<br /> | ||
==Overview== | ==Overview== | ||
| - | TEI-6720 (2-(3-cyano-4-isobutoxyphenyl)-4-methyl-5-thiazolecarboxylic | + | TEI-6720 (2-(3-cyano-4-isobutoxyphenyl)-4-methyl-5-thiazolecarboxylic acid) is an extremely potent inhibitor of xanthine oxidoreductase. Steady state kinetics measurements exhibit mixed type inhibition with K(i) and K(i)' values of 1.2 +/- 0.05 x 10(-10) m and 9 +/- 0.05 x 10(-10) m, respectively. Fluorescence-monitored titration experiments showed that TEI-6720 bound very tightly to both the active and the inactive desulfo-form of the enzyme. The dissociation constant determined for the desulfo-form was 2 +/- 0.03 x 10(-9) m; for the active form, the corresponding number was too low to allow accurate measurements. The crystal structure of the active sulfo-form of milk xanthine dehydrogenase complexed with TEI-6720 and determined at 2.8-A resolution revealed the inhibitor molecule bound in a long, narrow channel leading to the molybdenum-pterin active site of the enzyme. It filled up most of the channel and the immediate environment of the cofactor, very effectively inhibiting the activity of the enzyme through the prevention of substrate binding. Although the inhibitor did not directly coordinate to the molybdenum ion, numerous hydrogen bonds as well as hydrophobic interactions with the protein matrix were observed, most of which are also used in substrate recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 1N5X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with FES, MTE, MOS, FAD and TEI as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Xanthine_dehydrogenase Xanthine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.4 1.17.1.4] Full crystallographic information is available from [http:// | + | 1N5X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=FES:'>FES</scene>, <scene name='pdbligand=MTE:'>MTE</scene>, <scene name='pdbligand=MOS:'>MOS</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=TEI:'>TEI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Xanthine_dehydrogenase Xanthine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.4 1.17.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Xanthine dehydrogenase]] | [[Category: Xanthine dehydrogenase]] | ||
| - | [[Category: Eger, B | + | [[Category: Eger, B T.]] |
[[Category: Kondo, S.]] | [[Category: Kondo, S.]] | ||
[[Category: Nishino, T.]] | [[Category: Nishino, T.]] | ||
[[Category: Okamoto, K.]] | [[Category: Okamoto, K.]] | ||
| - | [[Category: Pai, E | + | [[Category: Pai, E F.]] |
[[Category: FAD]] | [[Category: FAD]] | ||
[[Category: FES]] | [[Category: FES]] | ||
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[[Category: tei-6720]] | [[Category: tei-6720]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:37 2008'' |
Revision as of 12:02, 21 February 2008
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Xanthine Dehydrogenase from Bovine Milk with Inhibitor TEI-6720 Bound
Overview
TEI-6720 (2-(3-cyano-4-isobutoxyphenyl)-4-methyl-5-thiazolecarboxylic acid) is an extremely potent inhibitor of xanthine oxidoreductase. Steady state kinetics measurements exhibit mixed type inhibition with K(i) and K(i)' values of 1.2 +/- 0.05 x 10(-10) m and 9 +/- 0.05 x 10(-10) m, respectively. Fluorescence-monitored titration experiments showed that TEI-6720 bound very tightly to both the active and the inactive desulfo-form of the enzyme. The dissociation constant determined for the desulfo-form was 2 +/- 0.03 x 10(-9) m; for the active form, the corresponding number was too low to allow accurate measurements. The crystal structure of the active sulfo-form of milk xanthine dehydrogenase complexed with TEI-6720 and determined at 2.8-A resolution revealed the inhibitor molecule bound in a long, narrow channel leading to the molybdenum-pterin active site of the enzyme. It filled up most of the channel and the immediate environment of the cofactor, very effectively inhibiting the activity of the enzyme through the prevention of substrate binding. Although the inhibitor did not directly coordinate to the molybdenum ion, numerous hydrogen bonds as well as hydrophobic interactions with the protein matrix were observed, most of which are also used in substrate recognition.
About this Structure
1N5X is a Single protein structure of sequence from Bos taurus with , , , and as ligands. Active as Xanthine dehydrogenase, with EC number 1.17.1.4 Full crystallographic information is available from OCA.
Reference
An extremely potent inhibitor of xanthine oxidoreductase. Crystal structure of the enzyme-inhibitor complex and mechanism of inhibition., Okamoto K, Eger BT, Nishino T, Kondo S, Pai EF, Nishino T, J Biol Chem. 2003 Jan 17;278(3):1848-55. Epub 2002 Nov 5. PMID:12421831
Page seeded by OCA on Thu Feb 21 14:02:37 2008
Categories: Bos taurus | Single protein | Xanthine dehydrogenase | Eger, B T. | Kondo, S. | Nishino, T. | Okamoto, K. | Pai, E F. | FAD | FES | MOS | MTE | TEI | 3d-structure | Fad | Fe2/s2 center | Flavoprotein | Iron-sulfur center | Molybdopterin | Oxidoreductase | Tei-6720
