1n6m
From Proteopedia
(New page: 200px<br /><applet load="1n6m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n6m, resolution 2.5Å" /> '''Rotation of the stalk...) |
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| - | [[Image:1n6m.gif|left|200px]]<br /><applet load="1n6m" size=" | + | [[Image:1n6m.gif|left|200px]]<br /><applet load="1n6m" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n6m, resolution 2.5Å" /> | caption="1n6m, resolution 2.5Å" /> | ||
'''Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd'''<br /> | '''Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Molecular motors undergo conformational changes to produce force and move | + | Molecular motors undergo conformational changes to produce force and move along cytoskeletal filaments. Structural changes have been detected in kinesin motors; however, further changes are expected because previous crystal structures are in the same or closely related conformations. We report here a 2.5 A crystal structure of the minus-end kinesin, Ncd, with the coiled-coil stalk/neck and one head rotated by approximately 75 degrees relative to the other head. The two heads are asymmetrically positioned with respect to the stalk and show asymmetry of nucleotide state: one head is fully occupied, but the other is unstably bound to ADP. Unlike previous structures, our new atomic model can be fit into cryoelectron microscopy density maps of the motor attached to microtubules, where it appears to resemble a one-head-bound motor with the stalk rotated towards the minus end. Interactions between neck and motor core residues, observed in the head that moves with the stalk, are disrupted in the other head, permitting rotation of the stalk/neck. The rotation could represent a force-producing stroke that directs the motor to the minus end. |
==About this Structure== | ==About this Structure== | ||
| - | 1N6M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1N6M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bronner, C | + | [[Category: Bronner, C E.]] |
| - | [[Category: Cha, S | + | [[Category: Cha, S S.]] |
| - | [[Category: Endow, S | + | [[Category: Endow, S A.]] |
| - | [[Category: Park, C | + | [[Category: Park, C G.]] |
| - | [[Category: Park, H | + | [[Category: Park, H W.]] |
[[Category: Yun, M.]] | [[Category: Yun, M.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
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[[Category: ncd]] | [[Category: ncd]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:50 2008'' |
Revision as of 12:02, 21 February 2008
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Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd
Overview
Molecular motors undergo conformational changes to produce force and move along cytoskeletal filaments. Structural changes have been detected in kinesin motors; however, further changes are expected because previous crystal structures are in the same or closely related conformations. We report here a 2.5 A crystal structure of the minus-end kinesin, Ncd, with the coiled-coil stalk/neck and one head rotated by approximately 75 degrees relative to the other head. The two heads are asymmetrically positioned with respect to the stalk and show asymmetry of nucleotide state: one head is fully occupied, but the other is unstably bound to ADP. Unlike previous structures, our new atomic model can be fit into cryoelectron microscopy density maps of the motor attached to microtubules, where it appears to resemble a one-head-bound motor with the stalk rotated towards the minus end. Interactions between neck and motor core residues, observed in the head that moves with the stalk, are disrupted in the other head, permitting rotation of the stalk/neck. The rotation could represent a force-producing stroke that directs the motor to the minus end.
About this Structure
1N6M is a Single protein structure of sequence from Drosophila melanogaster with and as ligands. Full crystallographic information is available from OCA.
Reference
Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd., Yun M, Bronner CE, Park CG, Cha SS, Park HW, Endow SA, EMBO J. 2003 Oct 15;22(20):5382-9. PMID:14532111
Page seeded by OCA on Thu Feb 21 14:02:50 2008
Categories: Drosophila melanogaster | Single protein | Bronner, C E. | Cha, S S. | Endow, S A. | Park, C G. | Park, H W. | Yun, M. | ADP | MG | Kinesin | Microtubule | Motor | Ncd
