1n7a
From Proteopedia
(New page: 200px<br /><applet load="1n7a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n7a, resolution 1.20Å" /> '''RIP-Radiation-damage...) |
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| - | [[Image:1n7a.gif|left|200px]]<br /><applet load="1n7a" size=" | + | [[Image:1n7a.gif|left|200px]]<br /><applet load="1n7a" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n7a, resolution 1.20Å" /> | caption="1n7a, resolution 1.20Å" /> | ||
'''RIP-Radiation-damage Induced Phasing'''<br /> | '''RIP-Radiation-damage Induced Phasing'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The use of third generation synchrotron sources has led to renewed concern | + | The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1N7A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K and SPM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1N7A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Caffrey, M.]] | [[Category: Caffrey, M.]] | ||
| - | [[Category: Leiros, H | + | [[Category: Leiros, H K.S.]] |
[[Category: McSweeney, S.]] | [[Category: McSweeney, S.]] | ||
[[Category: Pan, B.]] | [[Category: Pan, B.]] | ||
| - | [[Category: Ravelli, R | + | [[Category: Ravelli, R B.G.]] |
[[Category: K]] | [[Category: K]] | ||
[[Category: SPM]] | [[Category: SPM]] | ||
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[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:00 2008'' |
Revision as of 12:03, 21 February 2008
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RIP-Radiation-damage Induced Phasing
Overview
The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.
About this Structure
1N7A is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Specific radiation damage can be used to solve macromolecular crystal structures., Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S, Structure. 2003 Feb;11(2):217-24. PMID:12575941
Page seeded by OCA on Thu Feb 21 14:03:00 2008
Categories: Protein complex | Caffrey, M. | Leiros, H K.S. | McSweeney, S. | Pan, B. | Ravelli, R B.G. | K | SPM | Dna | Rna
