1n7s
From Proteopedia
(New page: 200px<br /><applet load="1n7s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n7s, resolution 1.45Å" /> '''High Resolution Stru...) |
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| - | [[Image:1n7s.gif|left|200px]]<br /><applet load="1n7s" size=" | + | [[Image:1n7s.gif|left|200px]]<br /><applet load="1n7s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n7s, resolution 1.45Å" /> | caption="1n7s, resolution 1.45Å" /> | ||
'''High Resolution Structure of a Truncated Neuronal SNARE Complex'''<br /> | '''High Resolution Structure of a Truncated Neuronal SNARE Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a truncated SNARE complex has been solved to 1.4-A | + | The structure of a truncated SNARE complex has been solved to 1.4-A resolution revealing a stabilizing salt bridge, sites of hydration, and conformational variability of the ionic central layer that were not observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid binding and denatures at a lower temperature than longer complexes as assessed by SDS and circular dichroism thermal melts. The truncated SNARE complex is monomeric, and it retains binding to synaptotagmin I. |
==About this Structure== | ==About this Structure== | ||
| - | 1N7S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1N7S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
| - | [[Category: Brunger, A | + | [[Category: Brunger, A T.]] |
| - | [[Category: Ernst, J | + | [[Category: Ernst, J A.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: MPD]] | [[Category: MPD]] | ||
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[[Category: neuronal snare protein complex]] | [[Category: neuronal snare protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:08 2008'' |
Revision as of 12:03, 21 February 2008
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High Resolution Structure of a Truncated Neuronal SNARE Complex
Overview
The structure of a truncated SNARE complex has been solved to 1.4-A resolution revealing a stabilizing salt bridge, sites of hydration, and conformational variability of the ionic central layer that were not observed in a previously published structure at 2.4-A resolution (Sutton, R. B., Fasshauer, D., Jahn, R., and Brunger, A. T. (1998) Nature 395, 347-353). The truncated complex lacks residues involved in phospholipid binding and denatures at a lower temperature than longer complexes as assessed by SDS and circular dichroism thermal melts. The truncated SNARE complex is monomeric, and it retains binding to synaptotagmin I.
About this Structure
1N7S is a Protein complex structure of sequences from Rattus norvegicus with and as ligands. Full crystallographic information is available from OCA.
Reference
High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex., Ernst JA, Brunger AT, J Biol Chem. 2003 Mar 7;278(10):8630-6. Epub 2002 Dec 20. PMID:12496247
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