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3eq4
From Proteopedia
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | [[3eq4]] is a 9 chain structure | + | [[3eq4]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQ4 OCA]. |
==See Also== | ==See Also== | ||
| + | *[[Elongation factor|Elongation factor]] | ||
*[[Ribosomal protein L11|Ribosomal protein L11]] | *[[Ribosomal protein L11|Ribosomal protein L11]] | ||
*[[Ribosomal protein S12|Ribosomal protein S12]] | *[[Ribosomal protein S12|Ribosomal protein S12]] | ||
| Line 16: | Line 17: | ||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID:019020518</ref><references group="xtra"/> | <ref group="xtra">PMID:019020518</ref><references group="xtra"/> | ||
| - | [[Category: Escherichia coli | + | [[Category: Escherichia coli k-12]] |
[[Category: Agirrezabala, X.]] | [[Category: Agirrezabala, X.]] | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
Revision as of 16:59, 20 October 2012
Contents |
Model of tRNA(Leu)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM
The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI). The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.
Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM., Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J, EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3eq4 is a 9 chain structure with sequence from Escherichia coli k-12. Full crystallographic information is available from OCA.
See Also
Reference
- Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J. Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM. EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518 doi:10.1038/emboj.2008.243
Categories: Escherichia coli k-12 | Agirrezabala, X. | Frank, J. | Li, W. | A/t-trna | Antibiotic resistance | Automated data collection | Elongation factor | Gtp-binding | Membrane | Methylation | Nucleotide-binding | Phosphoprotein | Protein biosynthesis | Protein translation | Ribonucleoprotein | Ribosomal protein | Ribosomal protein-rna complex | Rna-binding | Rrna-binding | Ternary complex | Trna-binding
