1n7j
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The crystal structures of human phenylethanolamine N-methyltransferase in | + | The crystal structures of human phenylethanolamine N-methyltransferase in complex with S-adenosyl-l-homocysteine (7, AdoHcy) and either 7-iodo-1,2,3,4-tetrahydroisoquinoline (2) or 8,9-dichloro-2,3,4,5-tetrahydro-1H-2-benzazepine (3, LY134046) were determined and compared with the structure of the enzyme complex with 7 and 7-aminosulfonyl-1,2,3,4-tetrahydroisoquinoline (1, SK&F 29661). The enzyme is able to accommodate a variety of chemically disparate functional groups on the aromatic ring of the inhibitors through adaptation of the binding pocket for this substituent and by subtle adjustments of the orientation of the inhibitors within the relatively planar binding site. In addition, the interactions formed by the amine nitrogen of all three inhibitors reinforce the hypothesis that this functional group mimics the beta-hydroxyl of norepinephrine rather than the amine. These studies provide further clues for the development of improved inhibitors for use as pharmacological probes. |
==Disease== | ==Disease== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Archbold, J.]] | [[Category: Archbold, J.]] | ||
| - | [[Category: Caine, J | + | [[Category: Caine, J M.]] |
| - | [[Category: Criscione, K | + | [[Category: Criscione, K R.]] |
| - | [[Category: Grunewald, G | + | [[Category: Grunewald, G L.]] |
| - | [[Category: Martin, J | + | [[Category: Martin, J L.]] |
| - | [[Category: McLeish, M | + | [[Category: McLeish, M J.]] |
| - | [[Category: McMillan, F | + | [[Category: McMillan, F M.]] |
[[Category: IDI]] | [[Category: IDI]] | ||
[[Category: SAH]] | [[Category: SAH]] | ||
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[[Category: s-adenosylmethionine]] | [[Category: s-adenosylmethionine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:04 2008'' |
Revision as of 12:03, 21 February 2008
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The structure of Phenylethanolamine N-methyltransferase in complex with S-adenosylhomocysteine and an iodinated inhibitor
Contents |
Overview
The crystal structures of human phenylethanolamine N-methyltransferase in complex with S-adenosyl-l-homocysteine (7, AdoHcy) and either 7-iodo-1,2,3,4-tetrahydroisoquinoline (2) or 8,9-dichloro-2,3,4,5-tetrahydro-1H-2-benzazepine (3, LY134046) were determined and compared with the structure of the enzyme complex with 7 and 7-aminosulfonyl-1,2,3,4-tetrahydroisoquinoline (1, SK&F 29661). The enzyme is able to accommodate a variety of chemically disparate functional groups on the aromatic ring of the inhibitors through adaptation of the binding pocket for this substituent and by subtle adjustments of the orientation of the inhibitors within the relatively planar binding site. In addition, the interactions formed by the amine nitrogen of all three inhibitors reinforce the hypothesis that this functional group mimics the beta-hydroxyl of norepinephrine rather than the amine. These studies provide further clues for the development of improved inhibitors for use as pharmacological probes.
Disease
Known diseases associated with this structure: Hypertension, essential, 145500 (1) OMIM:[171190]
About this Structure
1N7J is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Phenylethanolamine N-methyltransferase, with EC number 2.1.1.28 Full crystallographic information is available from OCA.
Reference
Molecular recognition of sub-micromolar inhibitors by the epinephrine-synthesizing enzyme phenylethanolamine N-methyltransferase., McMillan FM, Archbold J, McLeish MJ, Caine JM, Criscione KR, Grunewald GL, Martin JL, J Med Chem. 2004 Jan 1;47(1):37-44. PMID:14695818
Page seeded by OCA on Thu Feb 21 14:03:04 2008
Categories: Homo sapiens | Phenylethanolamine N-methyltransferase | Single protein | Archbold, J. | Caine, J M. | Criscione, K R. | Grunewald, G L. | Martin, J L. | McLeish, M J. | McMillan, F M. | IDI | SAH | Adrenaline | Catecholamine | Epinephrine | Methyltransferase | S-adenolsylhomocysteine | S-adenosylmethionine
