1n96
From Proteopedia
(New page: 200px<br /><applet load="1n96" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n96" /> '''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCT...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1n96.gif|left|200px]]<br /><applet load="1n96" size=" | + | [[Image:1n96.gif|left|200px]]<br /><applet load="1n96" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n96" /> | caption="1n96" /> | ||
'''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)'''<br /> | '''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of a cyclic oligonucleotide d<pCGCTCATT> has been | + | The solution structure of a cyclic oligonucleotide d<pCGCTCATT> has been determined by two-dimensional NMR spectroscopy and restrained molecular dynamics. Under the appropriate experimental conditions, this molecule self-associates, forming a symmetric dimer stabilized by four intermolecular Watson-Crick base pairs. The resulting four-stranded structure consists of two G:C:A:T tetrads, formed by facing the minor groove side of the Watson-Crick base-pairs. Most probably, the association of the base-pairs is stabilized by coordinating a Na(+) cation. This is the first time that this novel G:C:A:T tetrad has been found in an oligonucleotide structure. This observation increases considerably the number of sequences that may adopt a four-stranded architecture. Overall, the three-dimensional structure is similar to those observed previously in other quadruplexes formed by minor groove alignment of Watson-Crick base pairs. This resemblance strongly suggests that we may be observing a general motif for DNA-DNA recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 1N96 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1N96 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N96 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Escaja, N.]] | [[Category: Escaja, N.]] | ||
| - | [[Category: Gelpi, J | + | [[Category: Gelpi, J L.]] |
[[Category: Gonzalez, C.]] | [[Category: Gonzalez, C.]] | ||
[[Category: Orozco, M.]] | [[Category: Orozco, M.]] | ||
| Line 23: | Line 23: | ||
[[Category: unusual dna]] | [[Category: unusual dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:38 2008'' |
Revision as of 12:03, 21 February 2008
|
DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)
Overview
The solution structure of a cyclic oligonucleotide d<pCGCTCATT> has been determined by two-dimensional NMR spectroscopy and restrained molecular dynamics. Under the appropriate experimental conditions, this molecule self-associates, forming a symmetric dimer stabilized by four intermolecular Watson-Crick base pairs. The resulting four-stranded structure consists of two G:C:A:T tetrads, formed by facing the minor groove side of the Watson-Crick base-pairs. Most probably, the association of the base-pairs is stabilized by coordinating a Na(+) cation. This is the first time that this novel G:C:A:T tetrad has been found in an oligonucleotide structure. This observation increases considerably the number of sequences that may adopt a four-stranded architecture. Overall, the three-dimensional structure is similar to those observed previously in other quadruplexes formed by minor groove alignment of Watson-Crick base pairs. This resemblance strongly suggests that we may be observing a general motif for DNA-DNA recognition.
About this Structure
1N96 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:12733903
Page seeded by OCA on Thu Feb 21 14:03:38 2008
