1nb7
From Proteopedia
(New page: 200px<br /><applet load="1nb7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nb7, resolution 2.9Å" /> '''HC-J4 RNA polymerase ...) |
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| - | [[Image:1nb7.gif|left|200px]]<br /><applet load="1nb7" size=" | + | [[Image:1nb7.gif|left|200px]]<br /><applet load="1nb7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nb7, resolution 2.9Å" /> | caption="1nb7, resolution 2.9Å" /> | ||
'''HC-J4 RNA polymerase complexed with short RNA template strand'''<br /> | '''HC-J4 RNA polymerase complexed with short RNA template strand'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Several crystal structures of the hepatitis C virus NS5B protein | + | Several crystal structures of the hepatitis C virus NS5B protein (genotype-1b, strain J4) complexed with metal ions, single-stranded RNA or nucleoside-triphosphates have been determined. These complexes illustrate how conserved amino acid side-chains, together with essential structural features within the active site, control nucleotide binding and likely mediate de-novo initiation. The incoming nucleotide interacts with several basic residues from an extension on the NS5B fingers domain, a beta-hairpin from the NS5B thumb domain and the C-terminal arm. The modular, bi-partite fingers domain carries a long binding groove which guides the template towards the catalytic site. The apo-polymerase structure provides unprecedented insights into potential non-nucleoside inhibitor binding sites located between palm and thumb near motif E, which is unique to RNA polymerases and reverse transcriptases. |
==About this Structure== | ==About this Structure== | ||
| - | 1NB7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http:// | + | 1NB7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NB7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Farrell, D | + | [[Category: Farrell, D J.O.]] |
[[Category: Jaeger, J.]] | [[Category: Jaeger, J.]] | ||
| - | [[Category: Rowlands, D | + | [[Category: Rowlands, D J.]] |
[[Category: Trowbridge, R.]] | [[Category: Trowbridge, R.]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:11 2008'' |
Revision as of 12:04, 21 February 2008
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HC-J4 RNA polymerase complexed with short RNA template strand
Overview
Several crystal structures of the hepatitis C virus NS5B protein (genotype-1b, strain J4) complexed with metal ions, single-stranded RNA or nucleoside-triphosphates have been determined. These complexes illustrate how conserved amino acid side-chains, together with essential structural features within the active site, control nucleotide binding and likely mediate de-novo initiation. The incoming nucleotide interacts with several basic residues from an extension on the NS5B fingers domain, a beta-hairpin from the NS5B thumb domain and the C-terminal arm. The modular, bi-partite fingers domain carries a long binding groove which guides the template towards the catalytic site. The apo-polymerase structure provides unprecedented insights into potential non-nucleoside inhibitor binding sites located between palm and thumb near motif E, which is unique to RNA polymerases and reverse transcriptases.
About this Structure
1NB7 is a Single protein structure of sequence from Hepatitis c virus with as ligand. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
Substrate complexes of hepatitis C virus RNA polymerase (HC-J4): structural evidence for nucleotide import and de-novo initiation., O'Farrell D, Trowbridge R, Rowlands D, Jager J, J Mol Biol. 2003 Feb 28;326(4):1025-35. PMID:12589751
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