1nbw
From Proteopedia
(New page: 200px<br /><applet load="1nbw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nbw, resolution 2.4Å" /> '''Glycerol dehydratase ...) |
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| - | [[Image:1nbw.jpg|left|200px]]<br /><applet load="1nbw" size=" | + | [[Image:1nbw.jpg|left|200px]]<br /><applet load="1nbw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nbw, resolution 2.4Å" /> | caption="1nbw, resolution 2.4Å" /> | ||
'''Glycerol dehydratase reactivase'''<br /> | '''Glycerol dehydratase reactivase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The function of glycerol dehydratase (GDH) reactivase is to remove damaged | + | The function of glycerol dehydratase (GDH) reactivase is to remove damaged coenzyme B(12) from GDH that has suffered mechanism-based inactivation. The structure of GDH reactivase from Klebsiella pneumoniae was determined at 2.4 A resolution by the single isomorphous replacement with anomalous signal (SIR/AS) method. Each tetramer contains two elongated 63 kDa alpha subunits and two globular 14 kDa beta subunits. The alpha subunit contains structural features resembling both GroEL and Hsp70 groups of chaperones, and it appears chaperone like in its interactions with ATP. The fold of the beta subunit resembles that of the beta subunit of glycerol dehydratase, except that it lacks some coenzyme B(12) binding elements. A hypothesis for the reactivation mechanism of reactivase is proposed based on these structural features. |
==About this Structure== | ==About this Structure== | ||
| - | 1NBW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1NBW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dotson, G.]] | [[Category: Dotson, G.]] | ||
| - | [[Category: Jr., I | + | [[Category: Jr., I Turner.]] |
| - | [[Category: Liao, D | + | [[Category: Liao, D I.]] |
[[Category: Reiss, L.]] | [[Category: Reiss, L.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: reactivase]] | [[Category: reactivase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:24 2008'' |
Revision as of 12:04, 21 February 2008
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Glycerol dehydratase reactivase
Overview
The function of glycerol dehydratase (GDH) reactivase is to remove damaged coenzyme B(12) from GDH that has suffered mechanism-based inactivation. The structure of GDH reactivase from Klebsiella pneumoniae was determined at 2.4 A resolution by the single isomorphous replacement with anomalous signal (SIR/AS) method. Each tetramer contains two elongated 63 kDa alpha subunits and two globular 14 kDa beta subunits. The alpha subunit contains structural features resembling both GroEL and Hsp70 groups of chaperones, and it appears chaperone like in its interactions with ATP. The fold of the beta subunit resembles that of the beta subunit of glycerol dehydratase, except that it lacks some coenzyme B(12) binding elements. A hypothesis for the reactivation mechanism of reactivase is proposed based on these structural features.
About this Structure
1NBW is a Protein complex structure of sequences from Klebsiella pneumoniae with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of glycerol dehydratase reactivase: a new type of molecular chaperone., Liao DI, Reiss L, Turner I, Dotson G, Structure. 2003 Jan;11(1):109-19. PMID:12517345
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