1nbq
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Reovirus attachment to cells is mediated by the binding of viral | + | Reovirus attachment to cells is mediated by the binding of viral attachment protein sigma 1 to junctional adhesion molecule 1 (JAM1). The crystal structure of the extracellular region of human JAM1 (hJAM1) reveals two concatenated Ig-type domains with a pronounced bend at the domain interface. Two hJAM1 molecules form a dimer that is stabilized by extensive ionic and hydrophobic contacts between the N-terminal domains. This dimeric arrangement is similar to that observed previously in the murine homolog of JAM1, indicating physiologic relevance. However, differences in the dimeric structures of hJAM1 and murine JAM1 suggest that the interface is dynamic, perhaps as a result of its ionic nature. We demonstrate that hJAM1, but not the related proteins hJAM2 and hJAM3, serves as a reovirus receptor, which provides insight into sites in hJAM1 that likely interact with sigma 1. In addition, we present evidence that the previously reported structural homology between sigma 1 and the adenovirus attachment protein, fiber, also extends to their respective receptors, which form similar dimeric structures. Because both receptors are located at regions of cell-cell contact, this similarity suggests that reovirus and adenovirus use conserved mechanisms of entry and pathways of infection. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aurrand-Lions, M.]] | [[Category: Aurrand-Lions, M.]] | ||
| - | [[Category: Campbell, J | + | [[Category: Campbell, J A.]] |
| - | [[Category: Dermody, T | + | [[Category: Dermody, T S.]] |
| - | [[Category: Forrest, J | + | [[Category: Forrest, J C.]] |
| - | [[Category: Imhof, B | + | [[Category: Imhof, B A.]] |
| - | [[Category: Peters, T | + | [[Category: Peters, T R.]] |
| - | [[Category: Prota, A | + | [[Category: Prota, A E.]] |
[[Category: Schelling, P.]] | [[Category: Schelling, P.]] | ||
[[Category: Stehle, T.]] | [[Category: Stehle, T.]] | ||
| - | [[Category: Watson, M | + | [[Category: Watson, M J.]] |
[[Category: adhesion molecule]] | [[Category: adhesion molecule]] | ||
[[Category: immunoglobulin superfamily]] | [[Category: immunoglobulin superfamily]] | ||
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[[Category: tight junction formation]] | [[Category: tight junction formation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:24 2008'' |
Revision as of 12:04, 21 February 2008
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Crystal Structure of Human Junctional Adhesion Molecule Type 1
Overview
Reovirus attachment to cells is mediated by the binding of viral attachment protein sigma 1 to junctional adhesion molecule 1 (JAM1). The crystal structure of the extracellular region of human JAM1 (hJAM1) reveals two concatenated Ig-type domains with a pronounced bend at the domain interface. Two hJAM1 molecules form a dimer that is stabilized by extensive ionic and hydrophobic contacts between the N-terminal domains. This dimeric arrangement is similar to that observed previously in the murine homolog of JAM1, indicating physiologic relevance. However, differences in the dimeric structures of hJAM1 and murine JAM1 suggest that the interface is dynamic, perhaps as a result of its ionic nature. We demonstrate that hJAM1, but not the related proteins hJAM2 and hJAM3, serves as a reovirus receptor, which provides insight into sites in hJAM1 that likely interact with sigma 1. In addition, we present evidence that the previously reported structural homology between sigma 1 and the adenovirus attachment protein, fiber, also extends to their respective receptors, which form similar dimeric structures. Because both receptors are located at regions of cell-cell contact, this similarity suggests that reovirus and adenovirus use conserved mechanisms of entry and pathways of infection.
About this Structure
1NBQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human junctional adhesion molecule 1: implications for reovirus binding., Prota AE, Campbell JA, Schelling P, Forrest JC, Watson MJ, Peters TR, Aurrand-Lions M, Imhof BA, Dermody TS, Stehle T, Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5366-71. Epub 2003 Apr 15. PMID:12697893
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