1ne3
From Proteopedia
(New page: 200px<br /><applet load="1ne3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ne3" /> '''Solution structure of ribosomal protein S28E...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ne3.gif|left|200px]]<br /><applet load="1ne3" size=" | + | [[Image:1ne3.gif|left|200px]]<br /><applet load="1ne3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ne3" /> | caption="1ne3" /> | ||
'''Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH0256_1_68; Northeast Structural Genomics Target TT744'''<br /> | '''Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH0256_1_68; Northeast Structural Genomics Target TT744'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The ribosomal protein S28E from the archaeon Methanobacterium | + | The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes. Here we report the three-dimensional solution structure of S28E by NMR spectroscopy. S28E contains a globular region and a long C-terminal tail protruding from the core. The globular region consists of four antiparallel beta-strands that are arranged in a Greek-key topology. Unique features of S28E include an extended loop L2-3 that folds back onto the protein and a 12-residue charged C-terminal tail with no regular secondary structure and greater flexibility relative to the rest of the protein. The structural and surface resemblance to OB-fold family of proteins and the presence of highly conserved basic residues suggest that S28E may bind to RNA. A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1NE3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http:// | + | 1NE3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NE3 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Methanothermococcus thermolithotrophicus]] | [[Category: Methanothermococcus thermolithotrophicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
| - | [[Category: Cort, J | + | [[Category: Cort, J R.]] |
[[Category: Edwards, A.]] | [[Category: Edwards, A.]] | ||
[[Category: Kennedy, M.]] | [[Category: Kennedy, M.]] | ||
| - | [[Category: NESG, Northeast | + | [[Category: NESG, Northeast Structural Genomics Consortium.]] |
[[Category: Pineda-Lucena, A.]] | [[Category: Pineda-Lucena, A.]] | ||
| - | [[Category: Ramelot, T | + | [[Category: Ramelot, T A.]] |
[[Category: Wu, B.]] | [[Category: Wu, B.]] | ||
[[Category: Yee, A.]] | [[Category: Yee, A.]] | ||
| Line 30: | Line 30: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:05 2008'' |
Revision as of 12:05, 21 February 2008
|
Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH0256_1_68; Northeast Structural Genomics Target TT744
Overview
The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes. Here we report the three-dimensional solution structure of S28E by NMR spectroscopy. S28E contains a globular region and a long C-terminal tail protruding from the core. The globular region consists of four antiparallel beta-strands that are arranged in a Greek-key topology. Unique features of S28E include an extended loop L2-3 that folds back onto the protein and a 12-residue charged C-terminal tail with no regular secondary structure and greater flexibility relative to the rest of the protein. The structural and surface resemblance to OB-fold family of proteins and the presence of highly conserved basic residues suggest that S28E may bind to RNA. A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA.
About this Structure
1NE3 is a Single protein structure of sequence from Methanothermococcus thermolithotrophicus. Full crystallographic information is available from OCA.
Reference
Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum., Wu B, Yee A, Pineda-Lucena A, Semesi A, Ramelot TA, Cort JR, Jung JW, Edwards A, Lee W, Kennedy M, Arrowsmith CH, Protein Sci. 2003 Dec;12(12):2831-7. PMID:14627743
Page seeded by OCA on Thu Feb 21 14:05:05 2008
Categories: Methanothermococcus thermolithotrophicus | Single protein | Arrowsmith, C H. | Cort, J R. | Edwards, A. | Kennedy, M. | NESG, Northeast Structural Genomics Consortium. | Pineda-Lucena, A. | Ramelot, T A. | Wu, B. | Yee, A. | Beta protein | Nesg | Northeast structural genomics consortium | Ocsp | Protein structure initiative | Psi | Structural genomics
